MicroRNA-155-5p suppresses PD-L1 expression in lung adenocarcinoma
MicroRNA-155-5p suppresses PD-L1 expression in lung adenocarcinoma
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DOI：
10.1002/2211-5463.12853
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发表时间：
2020-04-22
期刊：
影响因子：
2.6
通讯作者：
Ji, Jiansong
中科院分区：
文献类型：
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作者：
Huang, Jiansheng;Weng, Qiaoyou;Ji, Jiansong
MiR-155- 5 p是一种关键的致癌microRNA，可维持免疫稳态并介导炎症和肿瘤发生之间的相互作用。程序性死亡配体-1（PD-L1）的高表达在肿瘤免疫耐受中也起着重要作用。本研究旨在探讨miR-155- 5 p与PD-L1在肺腺癌（LUAD）细胞A549和H1650中的关系。通过定量逆转录-聚合酶链反应（qRT-PCR）检测LUAD患者中miR-155- 5 p和PD-L1的表达水平，并使用miR-155- 5 p的模拟物模拟A549或H1650细胞中的表达增加。24小时后，我们通过qRT-PCR、蛋白质印迹和流式细胞术测量PD-L1水平。此外，我们使用TargetScan（）确定了PD-L1 3 '-UTR中可以与miR-155- 5 p结合的两个位点（5 '-AGCAUUA-3 '和5 '-GCAUUAA-3 '）。与正常组织相比，miR-155- 5 p在肿瘤组织中过表达（P = 0.0456），而PD-L1的表达无显著差异（P = 0.1349）。miR-155- 5 p与PD-L1表达水平呈负相关（r =-0.6409，P = 0.0459和r =-0.7544，P = 0.0117）。外源性过表达miR-155- 5 p可降低A549和H1650细胞PD-L1 mRNA、总蛋白和膜蛋白的表达水平（P <0. 05）。总之，我们的数据表明，miR-155- 5 p可能抑制LUAD中PD-L1的表达。
MiR-155-5p is a key oncogenic microRNA that maintains immune homeostasis and mediates cross-talk between inflammation and tumorigenesis. High expression of programmed death ligand-1 (PD-L1) also plays an important role in immune tolerance in tumors. The present study aimed to explore the relationship between miR-155-5p and PD-L1 in lung adenocarcinoma (LUAD) cells A549 and H1650. The expression levels of miR-155-5p and PD-L1 in LUAD patients were detected by a quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and mimics of miR-155-5p were used to model increased expression in A549 or H1650 cells. After 24 h, we measured levels of PD-L1 by qRT-PCR, western blotting and flow cytometry. In addition, we identified two sites in the PD-L1 3 '-UTR (5 '-AGCAUUA-3 ' and 5 '-GCAUUAA-3 ') that can be bound by miR-155-5p using TargetScan (). Compared to normal tissue, miR-155-5p was overexpressed in tumor tissue (P = 0.0456), whereas the expression of PD-L1 was not significantly different (P = 0.1349). The expression levels of miR-155-5p and PD-L1 were negatively correlated (r = -0.6409, P = 0.0459 and r = -0.7544, P = 0.0117). Exogenous overexpression of miR-155-5p decreased the mRNA, total protein and membrane protein expression levels of PD-L1 both in A549 and H1650 cells (P < 0.05). Taken together, our data suggest that miR-155-5p may suppress the expression of PD-L1 in LUAD.