Social Experience Interacts with Serotonin to Affect Functional Connectivity in the Social Behavior Network following Playback of Social Vocalizations in Mice
Social Experience Interacts with Serotonin to Affect Functional Connectivity in the Social Behavior Network following Playback of Social Vocalizations in Mice
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DOI：
10.1523/eneuro.0247-20.2021
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发表时间：
2021-03
期刊：
影响因子：
3.4
通讯作者：
Christopher L. Petersen;Sarah E. D. Davis;Bhumi Patel;L. Hurley
中科院分区：
文献类型：
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作者：
Christopher L. Petersen;Sarah E. D. Davis;Bhumi Patel;L. Hurley
摘要过去的社会经验会影响负责产生和解释当前行为的电路。社会行为网络（SBN）是一个候选的神经集成，以调查早期生活的社会隔离的后果。SBN通过其多个核之间的协调活动模式（功能连接）来解释和产生社会行为，例如发声。然而，SBN是相对未开发的小鼠发声处理。该系统是敏感的，过去的经验和神经支配的SBN的许多节点，因此，我们测试了是否5-羟色胺信号与社会经验的相互作用，以影响模式的立即早期基因（IEG; cFos）的诱导在男性SBN以下播放社会发声。在出生后18-24天，将雄性小鼠分为每笼三只小鼠的社会圈养或隔离圈养。在圈养治疗28-30天后，将小鼠分为三个药物治疗组之一：对照组，芬氟拉明（FEN;增加可用的5-羟色胺），或pCPA（耗尽可用的5-羟色胺），并暴露于60分钟的女性宽带发声（BBV）播放。FEN普遍增加了SBN内cFos免疫反应（IR）神经元的数量，但在社会隔离小鼠中的作用更为明显。尽管cFos免疫反应性普遍增加，但与社会饲养的小鼠相比，隔离小鼠的功能连接性、聚集性和模块性降低。这些结果与精神病理学患者的功能性连接障碍的观察结果类似，并表明早期的社会隔离调节了社交网络的突触能调节。
Abstract Past social experience affects the circuitry responsible for producing and interpreting current behaviors. The social behavior network (SBN) is a candidate neural ensemble to investigate the consequences of early-life social isolation. The SBN interprets and produces social behaviors, such as vocalizations, through coordinated patterns of activity (functional connectivity) between its multiple nuclei. However, the SBN is relatively unexplored with respect to murine vocal processing. The serotonergic system is sensitive to past experience and innervates many nodes of the SBN; therefore, we tested whether serotonin signaling interacts with social experience to affect patterns of immediate early gene (IEG; cFos) induction in the male SBN following playback of social vocalizations. Male mice were separated into either social housing of three mice per cage or into isolated housing at 18–24 d postnatal. After 28–30 d in housing treatment, mice were parsed into one of three drug treatment groups: control, fenfluramine (FEN; increases available serotonin), or pCPA (depletes available serotonin) and exposed to a 60-min playback of female broadband vocalizations (BBVs). FEN generally increased the number of cFos-immunoreactive (-ir) neurons within the SBN, but effects were more pronounced in socially isolated mice. Despite a generalized increase in cFos immunoreactivity, isolated mice had reduced functional connectivity, clustering, and modularity compared with socially reared mice. These results are analogous to observations of functional dysconnectivity in persons with psychopathologies and suggests that early-life social isolation modulates serotonergic regulation of social networks.