Immunostaining of Skeletal Tissues.
Immunostaining of Skeletal Tissues.
复制标题
骨骼组织的免疫染色。
DOI:
10.1007/978-1-4939-8997-3_25
复制
发表时间:
2019
期刊:
影响因子:
--
通讯作者:
Roelofs AJ
中科院分区:
文献类型:
--
作者:
Roelofs AJ
Immunohistochemistry (IHC) is a routinely used technique in clinical diagnosis of pathological conditions and in basic and translational research. It combines anatomical, immunological, and biochemical methods and relies on the specific binding of an antibody to an antigen. Using the technique with mineralized tissues is more challenging than with soft tissues. Demineralizing the samples allows for embedding in paraffin wax, and also facilitates cryosectioning. This chapter describes methods for IHC on formaldehyde-fixed, demineralized, paraffin-embedded, or frozen sections to detect antigens in skeletal tissues.
影响因子:
3.2
作者:
Davis, A. Sally;Richter, Anke;Taubenberger, Jeffery K.
通讯作者:
Taubenberger, Jeffery K.