Immunostaining of Skeletal Tissues.

Immunostaining of Skeletal Tissues.
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骨骼组织的免疫染色。

DOI:
10.1007/978-1-4939-8997-3_25
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发表时间:
2019
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Roelofs AJ
Roelofs AJ
中科院分区:
--
文献类型:
--
作者:
Roelofs AJ

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免疫组织化学(IHC)是病理条件的临床诊断以及基础和转化研究中常规使用的技术。它结合了解剖学、免疫学和生物化学方法,并依赖于抗体与抗原的特异性结合。将该技术用于矿化组织比用于软组织更具挑战性。使样品脱矿物质允许包埋在石蜡中,并且还促进冷冻切片。本章描述了在甲醛固定、脱矿物质、石蜡包埋或冷冻切片上进行IHC检测骨骼组织中抗原的方法。
Immunohistochemistry (IHC) is a routinely used technique in clinical diagnosis of pathological conditions and in basic and translational research. It combines anatomical, immunological, and biochemical methods and relies on the specific binding of an antibody to an antigen. Using the technique with mineralized tissues is more challenging than with soft tissues. Demineralizing the samples allows for embedding in paraffin wax, and also facilitates cryosectioning. This chapter describes methods for IHC on formaldehyde-fixed, demineralized, paraffin-embedded, or frozen sections to detect antigens in skeletal tissues.
DOI: 10.1369/0022155414531549
发表时间: 2014-06-01
影响因子: 3.2
作者:
Davis, A. Sally;Richter, Anke;Taubenberger, Jeffery K.
通讯作者: Taubenberger, Jeffery K.