Advantages of Pure Platelet-Rich Plasma Compared with Leukocyte- and Platelet-Rich Plasma in Treating Rabbit Knee Osteoarthritis.
Advantages of Pure Platelet-Rich Plasma Compared with Leukocyte- and Platelet-Rich Plasma in Treating Rabbit Knee Osteoarthritis.
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纯富血小板血浆与白细胞和富血小板血浆相比治疗兔膝骨关节炎的优势
DOI：
10.12659/msm.898218
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发表时间：
2016-04-17
期刊：
影响因子：
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通讯作者：
Zhang CQ
中科院分区：
文献类型：
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作者：
Yin WJ;Xu HT;Sheng JG;An ZQ;Guo SC;Xie XT;Zhang CQ
富含白细胞和血小板的血浆（L-PRP）中浓缩的白细胞可提供增加水平的促炎细胞因子，以激活NF-κB信号通路，抵消生长因子对骨关节炎软骨的有益作用。然而，到目前为止，没有相关的研究已经证实，在体内。制备自体L-PRP和纯富血小板血浆（P-PRP），测定其成分组成，并在前交叉韧带切断后4、5和6周关节内注射。腹腔注射咖啡酸苯乙酯（CAPE）抑制NF-κB活化。术后8周处死动物。采用酶联免疫吸附试验测定关节液中白细胞介素1β（IL-1β）和前列腺素E2（PGE 2）的浓度，采用印度墨水染色进行大体形态学评估，采用苏木精-伊红染色和甲苯胺蓝染色进行组织学评估。与L-PRP相比，P-PRP注射在预防软骨破坏、保护软骨基质以及降低IL-1β和PGE 2浓度方面取得了更好的结果。CAPE注射逆转了L-PRP注射后滑液中IL-1β和PGE 2浓度的升高，并将L-PRP注射的结局改善至与P-PRP注射相似的水平，而它们对P-PRP注射的治疗效果没有影响。L-PRP中浓缩的白细胞可释放增加水平的促炎细胞因子以激活NF-κB信号传导途径，从而抵消生长因子对骨关节炎软骨的有益作用，最终导致L-PRP治疗骨关节炎的疗效劣于P-PRP。
Concentrated leukocytes in leukocyte- and platelet-rich plasma (L-PRP) may deliver increased levels of pro-inflammatory cytokines to activate the NF-κB signaling pathway, to counter the beneficial effects of growth factors on osteoarthritic cartilage. However, to date no relevant studies have substantiated that in vivo. Autologous L-PRP and pure platelet-rich plasma (P-PRP) were prepared, measured for componential composition, and injected intra-articularly after 4, 5, and 6 weeks post-anterior cruciate ligament transection. Caffeic acid phenethyl ester (CAPE) was injected intraperitoneally to inhibit NF-κB activation. All rabbits were sacrificed after 8 weeks postoperative. Enzyme-linked immunosorbent assays were performed to determine interleukin 1β (IL-1β) and prostaglandin E2 (PGE2) concentrations in the synovial fluid, Indian ink staining was performed for gross morphological assessment, and hematoxylin and eosin staining and toluidine blue staining were performed for histological assessment. Compared with L-PRP, P-PRP injections achieved better outcomes regarding the prevention of cartilage destruction, preservation of cartilaginous matrix, and reduction of IL-1β and PGE2 concentrations. CAPE injections reversed the increased IL-1β and PGE2 concentrations in the synovial fluid after L-PRP injections and improved the outcome of L-PRP injections to a level similar to P-PRP injections, while they had no influence on the therapeutic efficacy of P-PRP injections. Concentrated leukocytes in L-PRP may release increased levels of pro-inflammatory cytokines to activate the NF-κB signaling pathway, to counter the beneficial effects of growth factors on osteoarthritic cartilage, and finally, result in a inferior efficacy of L-PRP to P-PRP for the treatment of osteoarthritis.