IMMUNOCYTOCHEMICAL LOCALIZATION OF NATIVE CHONDROITIN-SULFATE IN TISSUES AND CULTURED-CELLS USING SPECIFIC MONOCLONAL-ANTIBODY
IMMUNOCYTOCHEMICAL LOCALIZATION OF NATIVE CHONDROITIN-SULFATE IN TISSUES AND CULTURED-CELLS USING SPECIFIC MONOCLONAL-ANTIBODY
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DOI：
10.1016/0092-8674(84)90276-9
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发表时间：
1984-01-01
期刊：
影响因子：
64.5
通讯作者：
GEIGER, B
中科院分区：
文献类型：
--
作者：
AVNUR, Z;GEIGER, B
用单克隆抗体CS-56在鸡组织和细胞培养物中观察到细胞外基质（ECM）中含有硫酸软骨素的蛋白聚糖（CSPG）。各种来源的培养细胞含有致密的点状层的CSPG的基板和细胞表面上，通过免疫荧光和免疫金染色确定。在培养条件下，含CSPG的基质通常被排除在稳定的细胞-基质局部接触之外。底物附着的CSPG表现出显着的化学稳定性，但可以成功地去除链霉蛋白酶或软骨素酶ABC和AC。用CS-56抗体孵育活细胞导致表面CSPG聚集成斑块，表明表面结合的CSPG可自由地沿质膜沿着横向移动。CS-56抗体揭示的CSPG-含有ECM的独特性质及其与特定类型的细胞接触的关系进行了讨论。
Chondroitin-sulfate containing proteoglycan (CSPG) of the extracellular matrix (ECM)was visualized in chick tissues and cell cultures with a monoclonal antibody, CS-56. Cultured cells of various origins contained dense punctate layers of CSPG on both the substrate and the cell surface, as determined by immunofluorescent and immunogold staining. Under culture conditions the CSPG-containing matrix was usually excluded from stable cell-to-substrate focal contacts. The substrate-attached CSPG exhibited remarkable chemical stability but could be successfully removed by pronase or chondroitinases ABC and AC. Incubation of living cells with CS-56 antibodies resulted in the clustering of surface CSPG into patches, indicating that the surface-bound CSPG is free to move laterally along the plasma membrane. The unique properties of the CSPG-containing ECM revealed by CS-56 antibodies and their relationships to specific types of cell contacts are discussed.