Lamin A/C speckles mediate spatial organization of splicing factor compartments and RNA polymerase II transcription.
Lamin A/C speckles mediate spatial organization of splicing factor compartments and RNA polymerase II transcription.
复制标题
层固定A/C斑点介导剪接因子室和RNA聚合酶II转录的空间组织。
DOI：
10.1083/jcb.200204149
复制
发表时间：
2002-12-09
影响因子：
7.8
通讯作者：
Parnaik, Veena K
中科院分区：
文献类型：
--
作者：
Kumaran, R Ileng;Muralikrishna, Bhattiprolu;Parnaik, Veena K
The A-type lamins have been observed to colocalize with RNA splicing factors in speckles within the nucleus, in addition to their typical distribution at the nuclear periphery. To understand the functions of lamin speckles, the effects of transcriptional inhibitors known to modify RNA splicing factor compartments (SFCs) were examined. Treatment of HeLa cells with α-amanitin or 5,6-dichlorobenzimidazole riboside (DRB) inhibited RNA polymerase II (pol II) transcription and led to the enlargement of lamin speckles as well as SFCs. Removal of the reversible inhibitor DRB resulted in the reactivation of transcription and a rapid, synchronous redistribution of lamins and splicing factors to normal-sized speckles, indicating a close association between lamin speckles and SFCs. Conversely, the expression of NH2-terminally modified lamin A or C in HeLa cells brought about a loss of lamin speckles, depletion of SFCs, and down-regulation of pol II transcription without affecting the peripheral lamina. Our results suggest a unique role for lamin speckles in the spatial organization of RNA splicing factors and pol II transcription in the nucleus.
