IDENTIFICATION AND NUCLEOTIDE-SEQUENCE OF THE DELTA-LYSIN GENE, HLD, ADJACENT TO THE ACCESSORY GENE REGULATOR (AGR) OF STAPHYLOCOCCUS-AUREUS
IDENTIFICATION AND NUCLEOTIDE-SEQUENCE OF THE DELTA-LYSIN GENE, HLD, ADJACENT TO THE ACCESSORY GENE REGULATOR (AGR) OF STAPHYLOCOCCUS-AUREUS
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DOI：
10.1007/bf00259623
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发表时间：
1989-11-01
期刊：
影响因子：
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通讯作者：
ARVIDSON, S
中科院分区：
文献类型：
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作者：
JANZON, L;LOFDAHL, S;ARVIDSON, S
金黄色葡萄球菌染色体的辅助基因调节器 (agr) 位点中的 Tn551 插入突变导致至少七种细胞外毒素和酶的产生减少，同时蛋白 A 和凝固酶的产生增加（Recsei 等，1986）。与该基因座相邻，我们现在已经鉴定出另一个基因 hld，它被转录成 0.5kb RNA，编码葡萄球菌 δ-赖氨酸。在携带 agr 插入突变的菌株中，hld 的表达被完全抑制。与链特异性探针的杂交和引物延伸分析表明，hld 和 agr 以相反方向转录，起始位置相距 188 个核苷酸。 hld基因主要在指数后生长期表达，在指数生早期完全受到抑制。不同生长阶段hld mRNA半衰期的测定表明这种调节是在转录水平。
A Tn551 insertional mutation in the accessory gene regulator (agr) locus of the Staphylococcus aureus chromosome resulted in the decreased production of at least seven extracellular toxins and enzymes and a simultaneous increase in the production of protein A and coagulase (Recsei et al. 1986). Adjacent to this locus we have now indentified another gene, hld, transcribed into a 0.5kb RNA which codes for the staphylococcal delta-lysin. The expression of hld was totally repressed in a strain carrying the agr insertional mutation. Hybridization with strand-specific probes and primer extension analysis revealed that hld and agr are transcribed in opposite directions, starting 188 nucleotides apart. The hld gene is mainly expressed during the post-exponential growth phase and is totally repressed during early exponential growth. Determination of hld mRNA half-life in different growth phases indicated that this regulation is at the level of transcription.