Demonstration of Adiponectin Receptors 1 and 2 mRNA expression in human breast cancer cells
Demonstration of Adiponectin Receptors 1 and 2 mRNA expression in human breast cancer cells
复制标题
DOI：
10.1016/j.canlet.2006.10.006
复制
发表时间：
2007-06-08
期刊：
影响因子：
9.7
通讯作者：
Noguchi, Shinzaburo
中科院分区：
文献类型：
--
作者：
Takahata, Chie;Miyoshi, Yasuo;Noguchi, Shinzaburo
最近，我们发现低脂联素水平与乳腺癌风险增加显著相关。研究脂联素受体1（AdipoR 1）和受体2（AdipoR 2）在人乳腺上皮细胞和乳腺癌细胞中的表达，对于阐明脂联素是否直接作用于这些细胞具有重要意义。使用从人乳腺癌细胞系获得的RNA样品，通过RT-PCR测定脂联素、AdipoR 1和AdipoR 2 mRNA的表达（MCF-7、T47D、SKBR 3和MDA-MB 231）、HMEC（正常人乳腺上皮细胞的原代培养物），脂肪组织（腋窝）以及通过激光显微切割（LMD）从乳腺癌组织中选择性收集的乳腺癌细胞和正常乳腺上皮细胞。脂联素mRNA仅在脂肪组织中表达。另一方面，在所有四种乳腺癌细胞系、HMEC、脂肪组织以及通过LMD选择性收集的乳腺癌细胞和正常乳腺上皮细胞中观察到AdipoR 1和AdipoR 2 mRNA表达。此外，AdipoR 1和AdipoR 2在正常乳腺上皮细胞和乳腺癌细胞中的表达通过免疫组织化学证实。这些结果表明脂联素可能直接通过AdipoR 1和AdipoR 2受体调节正常乳腺上皮细胞和乳腺癌细胞的生长，并且低血清脂联素水平与高乳腺癌风险的关联可能至少部分地由脂联素对乳腺上皮细胞的直接作用来解释。(c)2006爱思唯尔爱尔兰有限公司保留所有权利。
Recently, we have shown that low adiponectin levels are significantly associated with an increased breast cancer risk. It seems to be very important to study the expression of adiponectin receptor 1 (AdipoR1) and receptor 2 (AdipoR2) in the human breast epithelial cells and breast cancer cells in order to clarify whether or not adiponectin exerts its effects directly on these cells. Expression of adiponectin, AdipoR1, and AdipoR2 mRNA was determined by RT-PCR assay using the RNA samples obtained from human breast cancer cell lines (MCF-7, T47D, SKBR3, and MDA-MB231), HMEC (primary culture of normal human mammary epithelial cells), adipose tissues (axilla) as well as breast cancer cells and normal breast epithelial cells selectively collected from breast cancer tissues by laser microdissection (LMD). Adiponectin mRNA expression was observed only in the adipose tissues. On the other hand, AdipoR1 and AdipoR2 mRNA expression was observed in all four breast cancer cell lines, HMEC, adipose tissues as well as breast cancer cells and normal breast epithelial cells selectively collected by LMD. In addition, AdipoR1 and AdipoR2 expression in both normal breast epithelial cells and breast cancer cells was confirmed by immunohistochemistry. These results suggest a possibility that adiponectin might modulate the growth of normal breast epithelial cells and breast cancer cells directly through AdipoR1 and AdipoR2 receptors, and that the association of low serum adiponectin levels with a high breast cancer risk might be explained, at least in part, by the direct effect of adiponectin on the breast epithelial cells. (c) 2006 Elsevier Ireland Ltd. All rights reserved.