The PILATUS 1M detector
The PILATUS 1M detector
复制标题
DOI：
10.1107/s0909049505038665
复制
发表时间：
2006-03-01
影响因子：
2.5
通讯作者：
Wagner, A
中科院分区：
文献类型：
--
作者：
Broennimann, C;Eikenberry, EF;Wagner, A
Pilatus 1M探测器是一种混合像素阵列探测器，具有100多万个像素，以单光子计数模式运行。该探测器专为大分子结晶学设计，是目前同步加速器使用的最大像素阵列探测器。它是一个模块化系统，由18个多芯片模块组成，占地21厘米x 24厘米。部件的设计以及探测器的制造，包括凸点粘合，都是在保罗谢勒研究所(PSI)进行的。使用单光子计数探测器进行蛋白质结晶学研究需要对硅传感器的电荷收集特性进行详细研究。18个模块并行读出，整帧读出时间为6.7毫秒。这使得晶体数据可以在样品连续旋转的精细切片模式下获得。该探测器在PSI瑞士光源的蛋白质晶体光束线X06SA上进行了几次实验。数据是在使用快门的传统振荡模式下以及在精细Phi切片模式下收集的。在对来自Taumatin晶体的数据进行所有必要的校正后，对常规数据的处理导致了令人满意的合并8.5%量级的R因子。这使得第一次能够使用硅像素探测器来确定大分子生物晶体的精细电子密度图。
The PILATUS 1M detector is a hybrid pixel array detector with over one million pixels that operate in single photon counting mode. The detector, designed for macromolecular crystallography, is the largest pixel array detector currently in use at a synchrotron. It is a modular system consisting of 18 multichip modules covering an area of 21 cm x 24 cm. The design of the components as well as the manufacturing of the detector including the bump-bonding was performed at the Paul Scherrer Institute ( PSI). The use of a single photon counting detector for protein crystallography requires detailed studies of the charge collection properties of the silicon sensor. The 18 modules are read out in parallel, leading to a full frame readout-time of 6.7 ms. This allows crystallographic data to be acquired in fine-phi-slicing mode with continuous rotation of the sample. The detector was tested in several experiments at the protein crystallography beamline X06SA at the Swiss Light Source at PSI. Data were collected both in conventional oscillation mode using the shutter, as well as in a fine-phi-slicing mode. After applying all the necessary corrections to data from a thaumatin crystal, the processing of the conventional data led to satisfactory merging R-factors of the order of 8.5%. This allows, for the first time, determination of a refined electron density map of a macromolecular biological crystal using a silicon pixel detector.