Drying cells for SEM, AFM and TEM by hexamethyldisilazane: A study on hepatic endothelial cells
Drying cells for SEM, AFM and TEM by hexamethyldisilazane: A study on hepatic endothelial cells
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DOI：
10.1046/j.1365-2818.1997.1940755.x
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发表时间：
1997-04-01
期刊：
影响因子：
--
通讯作者：
Wisse, E
中科院分区：
文献类型：
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作者：
Braet, F;deZanger, R;Wisse, E
临界点干燥（CPD）是扫描电镜（SEM）中常用的生物样品干燥方法，六甲基二硅氮烷（HMDS）蒸发干燥是一种较好的替代方法，但该方法应用较少。因此，我们重新评估HMDS干燥。培养的大鼠肝窦内皮细胞（LEC），具有脆弱的窗孔和筛板，进行CPD和HMDS干燥，并在扫描电子显微镜，原子力显微镜（AFM）和透射电子显微镜（TEM）评价。我们没有观察到两种方法之间的差异，关于细胞超微结构。与CPD相比，HMDS干燥只需几分钟，工作量更小，化学品成本低，不需要设备。我们得出结论，HMDS干燥的标本具有相同的质量CPD的。此外，该方法也被证明是有用的干燥的TEM和AFM的整体安装细胞。
Critical point drying (CPD) is a common method of drying biological specimens for scanning electron microscopy (SEM), Drying by evaporation of hexamethyldisilazane (HMDS) has been described as a good alternative, This method, however, is infrequently used. Therefore, we reassessed HMDS drying, Cultured rat hepatic sinusoidal endothelial cells (LEC), possessing fragile fenestrae and sieve plates, were subjected to CPD and HMDS drying and evaluated in the scanning electron microscope, atomic force microscope (AFM) and transmission electron microscope (TEM). We observed no differences between the two methods regarding cellular ultrastructure. In contrast with CPD, HMDS drying takes only a few minutes, less effort, low costs for chemicals and requires no equipment. We conclude that HMDS-dried specimens have equal quality to CPD ones. Furthermore, the method also proved useful for drying whole-mount cells for TEM and AFM.