Fengycin produced by Bacillus subtilis NCD-2 plays a major role in biocontrol of cotton seedling damping-off disease
Fengycin produced by Bacillus subtilis NCD-2 plays a major role in biocontrol of cotton seedling damping-off disease
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DOI：
10.1016/j.micres.2013.12.001
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发表时间：
2014-01-01
影响因子：
6.7
通讯作者：
Ma, Ping
中科院分区：
文献类型：
--
作者：
Guo, Qinggang;Dong, Weixin;Ma, Ping
枯草芽孢杆菌NCD-2菌株对植物病原真菌有较强的拮抗作用，是一种优良的棉花土传病害生防菌株。本研究旨在对NCD-2菌株产生的主要抑菌活性物质进行鉴定，并阐明其对棉花立枯病的抑制作用。NCD-2菌株及其脂肽提取物对立枯丝核菌的生长有较强的抑制作用。通过快速蛋白质液相色谱（FPLC）分离菌株NCD-2的脂肽，并通过基质辅助激光解吸/电离飞行时间质谱分析将抗真菌化合物鉴定为丰霉素同系物簇。通过在B中框内缺失丰霉素合成酶基因，获得丰霉素缺陷型突变体。枯草杆菌NCD-2。与野生型菌株相比，该突变株抑制红曲霉生长的能力有所下降。在离体条件下对棉花立枯病的抑制作用和在体内对棉花立枯病的抑制作用。研究表明，丰霉素缺陷突变体在棉花根际中的种群数量与野生型NCD-2菌株基本相同。然而，R. fengycindeficient突变体定殖的棉花根际中的solani是NCD-2野生型菌株定殖的棉花根际中的solani的两倍。本研究表明，丰霉素型脂肽是B产生的主要抗真菌活性物质。枯草杆菌NCD-2。这些化合物在限制R.在棉花根际和抑制棉花立枯病中的作用。(C)2013 Elsevier GmbH. All rights reserved.
Bacillus subtilis strain NCD-2 is strongly antagonistic toward phytopathogenic fungi, and functions as an excellent biocontrol agent for cotton soil-borne diseases. The aims of this study were to characterize the main active antifungal compound from strain NCD-2 and clarify its role in suppressing cotton damping-off disease. Strain NCD-2 and lipopeptide extract prepared from an NCD-2 culture strongly inhibited the growth of Rhizoctonia solani in vitro. The lipopeptides of strain NCD-2 were separated by fast protein liquid chromatography (FPLC) and the antifungal compound was identified as a cluster of fengycin homologs analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. A fengycin-deficient mutant was obtained by in-frame deletion of the fengycin synthetase gene in B. subtilis NCD-2. Compared with the wild-type strain, this mutant showed decreased abilities to inhibit the growth of R. solani in vitro and to suppress cotton damping-off disease in vivo. Studies showed that the population of fengycin-deficient mutant was almost same as that of the wild-type NCD-2 strain in the cotton rhizosphere. However, the population of R. solani in the cotton rhizosphere colonized by the fengycindeficient mutant was twice that in the cotton rhizosphere colonized by the NCD-2 wild-type strain. This study demonstrated that fengycin-type lipopeptides are the main antifungal active compounds produced by B. subtilis NCD-2. These compounds play a major role in restricting the population of R. solani in the cotton rhizosphere and in suppressing cotton damping-off disease. (C) 2013 Elsevier GmbH. All rights reserved.