Large-Scale and Rapid Preparation of Nanofibrous Meshes and Their Application for Drug-Loaded Multilayer Mucoadhesive Patch Fabrication for Mouth Ulcer Treatment
Large-Scale and Rapid Preparation of Nanofibrous Meshes and Their Application for Drug-Loaded Multilayer Mucoadhesive Patch Fabrication for Mouth Ulcer Treatment
复制标题
纳米纤维网的大规模快速制备及其在口腔溃疡治疗的载药多层粘膜贴片制造中的应用
DOI：
10.1021/acsami.9b10379
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发表时间：
2019
影响因子：
9.5
通讯作者：
Duan Bin
中科院分区：
文献类型：
--
作者：
Wei Liang;Wu Shaohua;Shi Wen;Aldrich Amy L;Kielian Tammy;Carlson Mark A;Sun Runjun;Qin Xiaohong;Duan Bin
静电纺丝提供了一种简单方便的方法来制造纳米纤维网。然而，目前的生物降解技术生产能力往往局限于实验室规模，不能满足实际应用的要求。在本研究中，我们开发了一种新型的基于双环狭缝的无针静电纺丝装置来制备载药纳米纤维网。与传统的单针静电纺丝机相比，我们的无针静电纺丝机可以显着提高静电纺丝机的生产率，因为在静电纺丝过程中同时形成多个射流。采用自行研制的无针静电纺丝装置，制备了不同浓度的姜黄素负载聚L-乳酸（PLLA）微网。我们系统地研究了载姜黄素的PLLA纳米纤维网的药物释放行为、抗氧化特性、抗炎属性和细胞毒性。此外，双层纳米纤维复合材料网成功地产生通过静电纺丝姜黄素负载PLLA溶液和双氯芬酸钠负载聚（环氧乙烷）溶液在预定的时间序列，这揭示了强大的抗菌性能。随后，通过将双层复合纳米纤维网与基于（羟丙基）甲基纤维素的粘膜粘附膜组合来组装新型粘膜粘附贴片。多层粘膜粘附贴片在猪颊粘膜上具有优异的粘附性能。总的来说，我们的双环狭缝式聚合器可以提供一种新的方法来快速生产大规模的载药纳米纤维网，以制造粘膜粘附贴片。所述多层粘膜粘附贴剂能够掺入具有不同作用靶标的多种药物，例如镇痛剂、抗炎剂和抗微生物化合物，用于口腔溃疡或其他口腔疾病治疗。
Electrospinning provides a simple and convenient method to fabricate nanofibrous meshes. However, the nanofiber productivity is often limited to the laboratory scale, which cannot satisfy the requirements of practical application. In this study, we developed a novel needleless electrospinning spinneret based on a double-ring slit to fabricate drug-loaded nanofibrous meshes. In contrast to the conventional single-needle electrospinning spinneret, our needless spinneret can significantly improve nanofiber productivity due to the simultaneous formation of multiple jets during electrospinning. Curcumin-loaded poly(l-lactic acid) (PLLA) nanofiber meshes with various concentrations and on the large scale were manufactured by employing our developed needleless spinneret-based electrospinning device. We systematically investigated the drug release behaviors, antioxidant properties, anti-inflammatory attributes, and cytotoxicity of the curcumin-loaded PLLA nanofibrous meshes. Furthermore, a bilayer nanofibrous composite mesh was successfully generated by electrospinning curcumin-loaded PLLA solution and diclofenac sodium loaded poly(ethylene oxide) solution in a predetermined time sequence, which revealed potent antibacterial properties. Subsequently, novel mucoadhesive patches were assembled by combining the bilayer composite nanofibrous meshes with (hydroxypropyl)methyl cellulose based mucoadhesive film. The multilayered mucoadhesive patch has excellent adhesion properties on the porcine buccal mucosa. Overall, our double-ring slit spinneret can provide a novel method to rapidly produce large-scale drug-loaded nanofibrous meshes to fabricate mucoadhesive patches. The multiple-layered mucoadhesive patches enable the incorporation of multiple drugs with different targets of action, such as analgesic, anti-inflammatory, and antimicrobial compounds, for mouth ulcer or other oral disease treatments.