Sealing 3D-printed parts to poly(dimethylsiloxane) for simple fabrication of Microfluidic devices
Sealing 3D-printed parts to poly(dimethylsiloxane) for simple fabrication of Microfluidic devices
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DOI：
10.1016/j.aca.2020.05.014
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发表时间：
2020-08-08
影响因子：
6.2
通讯作者：
Henry, Charles S.
中科院分区：
文献类型：
--
作者：
Carrell, Cody S.;McCord, Cynthia P.;Henry, Charles S.
微流体技术已经彻底改变了生物分析化学、细胞生物学和分子生物学领域。然而，微流体技术的进步常常受到费力、耗时且资源密集的制造方法的限制，最常见的是一种光刻技术。3D打印的出现帮助研究人员更快速且低成本地制造概念验证微流体器件，但存在分辨率差以及从封闭通道中去除未固化树脂的繁琐后处理问题。此外，通常需要定制的树脂和打印机来制造完全封闭的通道，这增加了制造的成本和复杂性。在这项工作中，我们展示了通过将带有开口通道的3D打印部件与聚二甲基硅氧烷（PDMS）共价密封来制造微流体器件的能力。开口通道比完全封闭的通道更容易打印，并且可以使用廉价且市售的立体光刻3D打印机和树脂进行打印。3D打印部件使用两种不同的技术与PDMS密封，PDMS是传统微流体制造中常用的基底。第一种方法是在通过等离子体处理与PDMS密封之前，用市售的硅酮喷雾对部件进行涂层。在第二种技术中，固化的甲基丙烯酸酯树脂在用（3 - 氨丙基）三乙氧基硅烷（APTES）进行硅烷化之后，再通过等离子体处理与PDMS结合。这两种方法都在两种基底之间形成了牢固的密封，这通过几种类型的微流体器件（包括液滴和梯度发生器）得到了证明。（C）2020年由爱思唯尔B.V.出版
Microfluidics has revolutionized the fields of bioanalytical chemistry, cellular biology, and molecular biology. Advancements in microfluidic technologies, however, are often limited by labor, time, and resource-intensive fabrication methods, most commonly a form of photolithography. The advent of 3D printing has helped researchers fabricate proof-of-concept microfluidics more rapidly and at lower costs but suffers from poor resolution and tedious post-processing to remove uncured resin from enclosed channels. Additionally, custom resins and printers are often needed to create entirely enclosed channels, which increases cost and complexity of fabrication. In this work we demonstrate the ability to create microfluidic devices by covalently sealing 3D-printed parts with open-faced channels to polydimethylsiloxane (PDMS). Open-faced channels are easier to print than fully enclosed channels and can be printed using an inexpensive and commercially available stereolithography 3D printer and resin. The 3D-printed parts are sealed to PDMS, a common substrate used in traditional microfluidic fabrication, using two different techniques. The first involves coating the part with a commercially available silicone spray before sealing to PDMS via plasma treatment. In the second technique, the cured methacrylate resin is silanized with (3-Aminopropyl)triethoxysilane (APTES) before binding to PDMS with plasma treatment. Both methods create a strong seal between the two substrates, which is demonstrated with several types of microfluidic devices including droplet and gradient generators. (C) 2020 Published by Elsevier B.V.