POTENTIAL USE OF OVUM PICK-UP FOR EMBRYO PRODUCTION AND BREEDING IN CATTLE
POTENTIAL USE OF OVUM PICK-UP FOR EMBRYO PRODUCTION AND BREEDING IN CATTLE
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DOI：
10.1016/0093-691x(94)90384-u
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发表时间：
1994-09-01
期刊：
影响因子：
2.8
通讯作者：
PIETERSE, MC
中科院分区：
文献类型：
--
作者：
KRUIP, TAM;BONI, R;PIETERSE, MC
研究了超声引导下经阴道穿刺卵巢卵泡收集牛未成熟卵母细胞的效果。我们进行了三个实验来研究穿刺对卵泡再生和收集的卵母细胞数量的影响。每周穿刺两次，间隔时间分别为3和4 d。卵母细胞成熟、受精并在体外发育，记录可移植胚胎的数量。奶牛的健康状况每天都要检查。试验1，在5个月的时间内，对10头奶牛进行穿刺，收集到的卵母细胞与1头公牛的精液受精。实验2采集1头12岁高系谱奶牛的卵母细胞，穿刺1个月孕奶牛。将老牛的卵母细胞与8头不同公牛的精液进行受精。在试验3中，对6头肉牛（n = 6）进行为期2个月的穿刺，用同一品种的2头不同公牛的精液与其中3头牛的卵母细胞受精。实验1中，每次穿刺14.5 +/- 0.4（平均+/- SEM）个卵泡，回收8.0 +/- 0.3（平均+/- SEM）个卵母细胞。平均16%的卵母细胞发育成可移植的胚胎，受孕率为40%。实验结果在不同月份之间没有差异，表明经阴道穿刺方法可以成功地使用5个月。临床和死后检查没有发现有害影响，品种、年龄或生殖状态似乎也没有影响结果。然而，在奶牛个体之间和奶牛/公牛组合之间观察到很大的差异。
The efficacy of transvaginal ultrasound-guided puncturing of ovarian follicles for collecting immature oocytes in cattle was studied. Three experiments were conducted to examine the effects of puncturing on follicle recruitment and on the number of oocytes collected. Puncture sessions were executed twice weekly at regular intervals of 3 and 4 d respectively. The oocytes were matured, fertilized and allowed to develop in vitro and the number of transferable embryos was recorded. The health of the cows was checked daily. In Experiment 1, dairy cows (n=10) were punctured over a period of 5 mo, and the collected oocytes were fertilized with the semen of 1 bull. In Experiment 2, oocytes were collected from one 12 year old high pedigree dairy cow and an one month pregnant cow were punctured. The oocytes of the old cow were fertilized with semen of 8 different bulls. In Experiment 3, beef cows (n = 6) were punctured over a 2 mo period and the semen of 2 different bulls of the same breed was used to fertilize the oocytes from 3 of these cows. In Experiment 1, 14.5 +/- 0.4 (mean +/- SEM) follicles were punctured per session, and 8.0 +/- 0.3 (mean +/- SEM) oocytes were recovered. A mean of 16% of the oocytes developed into transferable embryos with a pregnancy rate of 40%. The results did not differ between the months of the experiments, indicating that the transvaginal puncturing method can be used successfully over a 5 mo period. No detrimental effects were observed after clinical and post mortem examinations, nor did breed, age or reproductive status appear to affect the results. However, large differences were observed between individual cows and between cow/bull combinations.