Value of the 13C-Urea Breath Test for Detection of Gastric Helicobacter spp. Infection in Dogs Undergoing Endoscopic Examination
Value of the 13C-Urea Breath Test for Detection of Gastric Helicobacter spp. Infection in Dogs Undergoing Endoscopic Examination
复制标题
DOI：
10.1292/jvms.12-0528
复制
发表时间：
2013-08-01
影响因子：
1.2
通讯作者：
Tsujimoto, Hajime
中科院分区：
文献类型：
--
作者：
Kubota, Sanae;Ohno, Koichi;Tsujimoto, Hajime
使用红外光谱分析仪的尿素呼气测试（UBT）广泛用于非侵入性快速检测胃螺杆菌属。适用于人类，但不适用于兽医。本研究的主要目的是确定犬UBT的参考范围并评估其临床实用性。为了实现第一个目标，对 6 只健康的实验室比格犬进行了 UBT 和上消化道内窥镜检查。对胃窦、胃体和胃底的胃内镜活检样本进行螺杆菌检查。通过聚合酶链反应（PCR）检测、快速脲酶检测（RUT）、组织学和细胞学。给感染的狗服用阿莫西林、甲硝唑和奥美拉唑14天，并用幽门螺杆菌阴性的狗来确定UBT的参考范围。为了实现第二个目标，32名犬患者在上消化道内镜检查前接受了UBT，并根据我们新确定的参考范围计算UBT的敏感性和特异性，使用PCR作为检测螺杆菌属的金标准。最初，所有 6 只实验室比格犬的所有胃区均受到感染，并在根除后不再受感染。根除后的平均+/- 2 SD UBT值为0.6 +/- 1.8%，并且UBT的参考范围被确定为小于千分之2.5。 27 名患者成功完成 UBT。使用我们的参考范围，UBT 显示出 89% (16/18) 的敏感性和 89% (8/9) 的特异性，表明 UBT 对于检测胃螺杆菌属非常有用。狗的感染。
Urea breath test (UBT) using an infrared spectral analyzer is widely used for non-invasive and rapid detection of gastric Helicobacter spp. in human, but not veterinary medicine. The main purposes of this study were to determine the reference range of the UBT in dogs and to evaluate its clinical usefulness. To address the first aim, 6 healthy laboratory beagles were subjected to UBT and upper gastrointestinal endoscopy. Gastric endoscopic biopsy samples from the antrum, corpus and fundus were examined for Helicobacter spp. by polymerase chain reaction (PCR) testing, rapid urease test (RUT), histology and cytology. Amoxicillin, metronidazole and omeprazole were given to infected dogs for 14 days, and dogs that became Helicobacter-negative were used to determine the reference range for UBT. To address the second aim, 32 canine patients underwent UBT before upper gastrointestinal endoscopy, and the sensitivity and specificity of UBT were calculated based on our newly determined reference range using PCR as the gold standard for detection of Helicobacter spp. Initially, all 6 laboratory beagles were infected in all gastric regions and became uninfected after eradication. The mean +/- 2 SD UBT value after eradication was 0.6 +/- 1.8%o, and the reference range for UBT was determined to be less than 2.5 parts per thousand. UBT was completed successfully in 27 patients. Using our reference range, UBT displayed 89% (16/18) sensitivity and 89% (8/9) specificity, indicating that UBT was quite useful for the detection of gastric Helicobacter spp. infection in dogs.