Demonstration of the retrograde transport of nerve growth factor receptor in the peripheral and central nervous system
Demonstration of the retrograde transport of nerve growth factor receptor in the peripheral and central nervous system
复制标题
神经生长因子受体在周围和中枢神经系统逆行转运的演示
作者：
E. Johnson;M. Taniuchi;H. Clark;J. Springer;S. Koh;M. W. Tayrien;R. Loy
神经生长因子通过与轴突末端的特异性神经生长因子受体结合作用于反应性神经元，然后将关键的生物化学信号逆行转运至细胞体。信号的身份是未知的;候选者包括NGF本身或其他一些“第二信使”。一个可能的第二信使是NGF受体。作为评估NGF受体在产生NGF依赖性信号中的可能作用以及理解NGF受体合成和利用的经济性的第一步，我们确定了NGF受体是否被逆行转运。使用单克隆抗体（192-IgG）对大鼠神经生长因子受体的免疫组化染色，我们寻找的积累神经生长因子受体分子的远端（逆行运输），以及近端（顺行运输），轴突结扎或横断的网站。到10-12小时，在结扎的坐骨神经和损伤的穹窿海马伞中，在结扎/损伤部位的近端和远端检测到积聚的NGF受体。转运受体可能位于交感神经和感觉神经元的坐骨神经和前脑胆碱能神经元从内侧隔投射到海马。在这两个解剖部位，近端（顺行）侧的神经生长因子受体的积累发生在细轴突过程的流，而染色的远端（逆行）侧发生在静脉曲张或颗粒状配置。这些结果提出了一种可能性，即神经生长因子受体在神经生长因子机制中的作用超出了轴突末端质膜的初始结合事件。
NGF acts on responsive neurons by binding to specific NGF receptors on axonal termini, after which a critical biochemical signal is retrogradely transported to the cell body. The identity of the signal(s) is unknown; candidates include NGF itself or some other “second messenger.” A possible second messenger is the NGF receptor. As a first step in assessing the possible role of NGF receptor in the generation of the NGF-dependent signal, and in understanding the economy of NGF receptor synthesis and utilization, we determined whether the NGF receptor is retrogradely transported. Using immunohistochemical staining with a monoclonal antibody (192-IgG) against rat NGF receptor, we looked for accumulation of NGF receptor molecules distal (retrograde transport), as well as proximal (anterograde transport), to sites of axonal ligation or transection. By 10–12 hr in both the ligated sciatic nerve and the lesioned fimbria- fornix, accumulated NGF receptor was detected proximal and distal to the ligation/lesion site. The transported receptor presumably was located in sympathetic and sensory neurons in the sciatic nerve and in forebrain cholinergic neurons projecting from the medial septum to the hippocampus. In both anatomical sites, accumulation of NGF receptor on the proximal (anterograde) side occurred in streams of fine axonal processes, whereas staining on the distal (retrograde) side occurred in varicose or granular configurations. These results raise the possibility that the NGF receptor has a role in the mechanism of NGF beyond the initial binding event at the plasma membrane of the axonal terminus.
单克隆抗体调节神经生长因子与 PC12 细胞的相互作用。
DOI：
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发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Chandler,CE;Parsons,LM;Hosang,M;Shooter,EM
通讯作者：
Shooter,EM
抑制表皮生长因子结合的单克隆抗体对表皮生长因子在不同细胞中的生物作用具有相反的作用。
DOI：
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发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Chandler,LP;Chandler,CE;Hosang,M;Shooter,EM
通讯作者：
Shooter,EM