Dynamic adsorption and dilatational properties of BSA at oil/water interface: Role of conformational flexibility
Dynamic adsorption and dilatational properties of BSA at oil/water interface: Role of conformational flexibility
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BSA 在油/水界面的动态吸附和膨胀特性：构象灵活性的作用
DOI：
10.1016/j.foodhyd.2014.06.014
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发表时间：
2015
影响因子：
10.7
通讯作者：
Lan Shen
中科院分区：
文献类型：
--
作者：
Chuan-He Tang;Lan Shen
采用液滴形状分析方法，系统研究了蛋白质浓度(C)和(或)二硫键(S-S)与β-巯基乙醇(2-ME)裂解对牛血清白蛋白(BSA)界面吸附动力学(包括扩散、穿透和界面结构重排)以及牛血清白蛋白动态膨胀特性的影响。结果表明，对于天然牛血清白蛋白，从0.01%增加到0.5%(w/v)，扩散速率和平衡表面压(π)逐渐提高，而InC(增加到1.0%，w/v)则相反地削弱了这些特性；增加表面压力，逐渐改善了界面的穿透性和结构重排。相反，与π相比，表面扩张模数(E)的发展对温度的依赖性较小。另一方面，在任何试验值下，S-S与2-ME的裂解都逐渐促进了扩散和渗透，以及“平衡”π，而结构重排高度依赖于c。当浓度为0.5%(w/v)时，随着2-ME浓度的增加，重排逐渐增强，而当浓度为0.5%或以上时，则呈现相反的趋势。S-S解理对整个吸附过程的弹性发展有普遍的促进作用，与C无关。E-π图表明，除了吸附作用外，不同浓度的Incand/或2-ME对界面的侧向大分子相互作用也有影响。牛血清白蛋白的构象柔韧性与界面特性、界面性质与乳化性能之间的关系已经得到了很好的研究。
The influence of protein concentration (c) and/or disulfide bond (S–S) cleavage withβ-mercaptoethanol (2-ME) on interfacial adsorption dynamics, including diffusion, penetration and structural rearrangement at the interface, as well as dynamic dilatational properties of bovine serum albumin (BSA) was systematically investigated using drop shape analysis. The results indicated that for native BSA increasing thecfrom 0.01 to 0.5% (w/v) progressively improved the rate of diffusion and the ‘equilibrium’ surface pressure (π), while a further increase inc(to 1.0%, w/v) contrarily impaired these characteristics; increasing thecprogressively improved the penetration and structural rearrangement at the interface. In contrast, the surface dilatational modulus (E) development was less dependent on thecthan that for theπ. On the other hand, the S–S cleavage with 2-ME at any testcvalue progressively improved the diffusion and penetration, as well as ‘equilibrium’π, while the structural rearrangement was highly dependent on thec. Atc< 0.5% (w/v), increasing the 2-ME concentration progressively improved the rearrangement, while atcvalues of 0.5% or above, a contrary trend was observed. The S–S cleavage generally improved theEor its elasticity development over the whole adsorption, regardless of thec. TheE-πplots indicated that besides the adsorption, the lateral macromolecular interactions at the interface could be affected by variation incand/or 2-ME concentration. The relationships between the conformational flexibility and interfacial characteristics of BSA, as well as between its interfacial and emulsifying properties have been well established.
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DOI：
10.1016/0927-7757(96)03534-0
发表时间：
1996-08
期刊：
Colloids and Surfaces A: Physicochemical and Engineering Aspects
影响因子：
--
作者：
A. Williams;A. Prins
通讯作者：
A. Williams;A. Prins
DOI：
10.1063/1.1724152
发表时间：
1946-05
期刊：
The Journal of physical and colloid chemistry
影响因子：
--
作者：
M. Wales;J. W. Williams
通讯作者：
M. Wales;J. W. Williams
DOI：
10.1016/s1359-0294(96)80077-x
发表时间：
1996-12
影响因子：
8.9
作者：
D. S. Home
通讯作者：
D. S. Home
DOI：
10.1016/0927-7765(93)01116-9
发表时间：
1994-09
期刊：
Colloids and Surfaces B: Biointerfaces
影响因子：
--
作者：
E. Dickinson;Y. Matsumura
通讯作者：
E. Dickinson;Y. Matsumura
影响因子：
10.7
作者：
V. P. Ruiz-Henestrosa;C. Sánchez;J. Pedroche;F. Millán;J. Patino
通讯作者：
V. P. Ruiz-Henestrosa;C. Sánchez;J. Pedroche;F. Millán;J. Patino