Optical Coherence Tomography
Optical Coherence Tomography
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DOI：
10.1007/978-3-540-46022-0_13
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发表时间：
2020-12
期刊：
影响因子：
--
通讯作者：
S. Boppart
中科院分区：
文献类型：
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作者：
S. Boppart
光学相干断层扫描 (OCT) 是一种新兴的成像技术，适用于广泛的生物、医学和材料研究 [1,2]。 OCT 在显微镜应用中的进步为研究人员提供了一种对生物样本和非生物样本进行可视化的新方法。 OCT 最初是为生物组织成像而开发的，因为它允许对组织微观结构进行原位成像，产生微米级图像分辨率，而无需切除样本进行组织处理。 OCT 类似于超声 B 模式成像，不同之处在于它使用低相干光而不是高频声音，并通过测量来自组织结构的光的反向散射强度来进行成像。 OCT 可以对组织或样本的横截面进行成像，如超声波中常见的操作，或内切面（如共焦和多光子显微镜中的操作）。 OCT图像是反向散射光强度的灰度或假彩色多维表示，表示微米尺度上不同组织类型之间的差异反向散射对比度。由于 OCT 使用光进行成像，因此其空间分辨率比超声波高一到两个数量级，并且不需要与标本或样品接触。光的使用还可以对组织和细胞结构进行光谱表征。
Optical coherence tomography (OCT) is an emerging imaging technique for a wide range of biological, medical, and material investigations [1,2]. Advances in the use of OCT for microscopy applications have provided researchers with a novel means by which biological specimens and non-biological samples are visualized. OCT was initially developed for imaging biological tissue because it permits the imaging of tissue microstructurein situ, yielding micron-scale image resolution without the need for excision of a specimen for tissue processing. OCT is analogous to ultrasound B-mode imaging except that it uses low-coherence light rather than high-frequency sound and performs imaging by measuring the backscattered intensity of light from structures in tissue. OCT can image tissue or specimens in cross-section, as is commonly done in ultrasound, or inen facesections, as in con-focal and multi-photon microscopy. The OCT image is a gray-scale or false-color multi-dimensional representation of backscattered light intensity that represents the differential backscattering contrast between different tissue types on a micron scale. Because OCT performs imaging using light, it has a one to two order-of-magnitude higher spatial resolution than ultrasound and does not require contact with the specimen or sample. The use of light also enables the spectroscopic characterization of tissue and cellular structures.