A MANUAL METHOD FOR THE SPECTROPHOTOMETRIC DETERMINATION OF REDUCING CARBOHYDRATES WITH 2-CYANOACETAMIDE
A MANUAL METHOD FOR THE SPECTROPHOTOMETRIC DETERMINATION OF REDUCING CARBOHYDRATES WITH 2-CYANOACETAMIDE
复制标题
DOI：
10.1016/0003-2697(82)90685-6
复制
发表时间：
1982-01-01
影响因子：
2.9
通讯作者：
KAKEHI, K
中科院分区：
文献类型：
--
作者：
HONDA, S;NISHIMURA, Y;KAKEHI, K
将D-葡萄糖的样品溶液（1 ml）、1%的2-氰基乙酰胺水溶液（1 ml）和0.1 M硼酸盐缓冲液（pH 9.0，2 ml）混合，并在沸水浴中加热10分钟。在276 nm处测量反应混合物的吸光度，可以快速和简单地测定5-750 nmol的D-葡萄糖，误差和变异系数分别小于0.4%和2.6%。其他还原性碳水化合物也有类似的结果。各种碳水化合物的相对摩尔吸光度进行了比较，并讨论了它们的结构。一些生化化合物的干扰也进行了研究。
A sample solution (1 m) of D-glucose, a 1% aqueous solution (1 ml) of 2-cyanoacetamide, and 0.1 M borate buffer (pH 9.0, 2 ml) are mixed and heated for 10 min in a boiling-water bath. Measurement of the absorbance of the reaction mixture at 276 nm allows the rapid and simple determination of 5-750 nmol of D-glucose with errors and coefficients of variation less than 0.4 and 2.6%, respectively. Other reducing carbohydrates give similar results. Relative molar absorbances for various carbohydrates are compared and discussed in relation to their structures. Interference with some biochemical compounds is also investigated.