AGO1 homeostasis entails coexpression of MIR168 and AGO1 and preferential stabilization of miR168 by AGO1
AGO1 homeostasis entails coexpression of MIR168 and AGO1 and preferential stabilization of miR168 by AGO1
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DOI：
10.1016/j.molcel.2006.03.011
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发表时间：
2006-04-07
期刊：
影响因子：
16
通讯作者：
Bartel, DP
中科院分区：
文献类型：
--
作者：
Vaucheret, H;Mallory, AC;Bartel, DP
拟南芥ARGONAUTE1（AGO1）编码microRNA（miRNA）途径的RNA切割酶，并受miR 168编程的AGO1催化的mRNA切割调节。在这里，我们描述了两个额外的调节过程所需的AGO1的稳态：转录共调节的MIR168和AGO1基因，和转录后稳定的MIR168 AGO1。通过使用突变或转基因破坏这些调节过程中的任何一个都会干扰miRNA途径的正常功能。相比之下，微小的扰动导致miR168和AGO1水平的微调转录后调节，从而维持其他miRNA的适当平衡，这些miRNA与AGO1一起控制miRNA靶点的mRNA水平。我们认为miR168的稳定发生在沉默复合物组装的水平上，并且调节组装miRNA程序化沉默复合物的效率在其他情况下也很重要。
Arabidopsis ARGONAUTE1 (AGO1) encodes the RNA slicer enzyme of the microRNA (miRNA) pathway and is regulated by miR168-programmed, AGO1-catalyzed mRNA cleavage. Here, we describe two additional regulatory processes required for AGO1 homeostasis: transcriptional coregulation of MIR168 and AGO1 genes, and posttranscriptional stabilization of miR168 by AGO1. Disrupting any of these regulatory processes by using mutations or transgenes disturbs a proper functioning of the miRNA pathway. In contrast, minor perturbation leads to fine-tuned posttranscriptional adjustment of miR168 and AGO1 levels, thereby maintaining a proper balance of other miRNAs, which, together with AGO1, control the mRNA levels of miRNA targets. We suggest that miR168 stabilization occurs at the level of silencing-complex assembly and that modulating the efficiency of assembling miRNA-programmed silencing complexes will also be important in other contexts.