Nanocurcumin restores aberrant miRNA expression profile in multiple sclerosis, randomized, double-blind, placebo-controlled trial
Nanocurcumin restores aberrant miRNA expression profile in multiple sclerosis, randomized, double-blind, placebo-controlled trial
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DOI：
10.1002/jcp.26301
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发表时间：
2018-07-01
影响因子：
5.6
通讯作者：
Yousefi, Mehdi
中科院分区：
文献类型：
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作者：
Dolati, Sanam;Aghebati-Maleki, Leili;Yousefi, Mehdi
在当前的研究中，我们的目的是确定纳米姜黄素对复发缓解型多发性硬化症 (RRMS) 患者外周血中 microRNA (miRNA) 的影响。我们打算在体内实验环境中研究这些 miRNA 的表达模式。对 50 名 RRMS 患者和 35 名健康对照者中选定的 27 种已知参与免疫反应调节的 miRNA 的表达水平进行了分析。通过定量 PCR (qPCR) 在基线和纳米姜黄素治疗 6 个月后研究 miRNA 表达谱。我们的数据显示，许多 microRNA 的表达，包括 miR-16、miR-17-92、miR-27、miR-29b、miR-126、miR-128、miR-132、miR-155、miR-326、miR-550、miR-15a、miR-19b、miR-106b、miR-320a、 miR-363、miR-31、miR-150 和 miR-340 受以下调节： 纳米姜黄素。目前的工作结果表明，纳米姜黄素能够恢复多发性硬化症患者失调的 miRNA 的表达模式。我们发现，与健康对照和接受纳米姜黄素治疗的患者相比，未经治疗的患者中的一些 miRNA 失调。这是一项新发现，可能代表这些 miRNA 对 MS 发病机制的潜在贡献。总而言之，这些数据为多发性硬化症中 B 细胞和 T 细胞功能的 miRNA 依赖性调节提供了新的见解，并丰富了我们对针对 B 细胞和 T 细胞的治疗方法介导的效应的理解。 在当前的研究中，我们的目的是确定纳米姜黄素对复发缓解型多发性硬化症 (RRMS) 患者外周血中 microRNA (miRNA) 的影响。我们打算在体内实验环境中研究这些 miRNA 的表达模式。
In the current study, we aimed to identify nanocurcumin effects on microRNAs (miRNAs) in the peripheral blood of patients with relapsing-remitting multiple sclerosis (RRMS). We intended to investigate the expression pattern of these miRNAs in experimental settings in vivo. The expression levels of the selected 27 miRNAs known to be involved in the regulation of immune responses were analyzed in 50 RRMS patients and 35 healthy controls. The miRNA expression profiles were investigated by quantitative PCR (qPCR) at baseline and after 6 months of nanocurcumin therapy. Our data revealed that the expression of a number of microRNAs including miR-16, miR-17-92, miR-27, miR-29b, miR-126, miR-128, miR-132, miR-155, miR-326, miR-550, miR-15a, miR-19b, miR-106b, miR-320a, miR-363, miR-31, miR-150, and miR-340 is regulated by nanocurcumin. The results of the current work indicate that nanocurcumin is able to restore the expression pattern of dysregulated miRNAs in MS patients. We discovered that some miRNAs are deregulated in untreated patients compared with healthy controls and nanocurcumin-treated patients. This is a new finding that might represent the potential contribution of these miRNAs to MS pathogenesis. Taken together, these data provide novel insights into miRNA-dependent regulation of the function of B and T cells in MS disease and enrich our understanding of the effects mediated by a therapeutic approach that targets B and T cells.In the current study, we aimed to identify nanocurcumin effects on microRNAs (miRNAs) in the peripheral blood of patients with relapsing-remitting multiple sclerosis (RRMS). We intended to investigate the expression pattern of these miRNAs in experimental settings in vivo.