Genesis of and trafficking to the Maurer's clefts of Plasmodium falciparum-infected erythrocytes
Genesis of and trafficking to the Maurer's clefts of Plasmodium falciparum-infected erythrocytes
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DOI：
10.1128/mcb.00095-06
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发表时间：
2006-06-01
影响因子：
5.3
通讯作者：
Tilley, Leann
中科院分区：
文献类型：
--
作者：
Spycher, Cornelia;Rug, Melanie;Tilley, Leann
疟疾寄生虫将蛋白质输出到它们自身质膜之外的红细胞中。Maurer's cleft是宿主细胞质内寄生虫衍生的结构，被认为是寄生虫和红细胞膜之间的分选室。然而，这个隔间的起源和将蛋白质引导到毛雷尔裂隙的信号尚不清楚。我们已经产生了恶性疟原虫感染的红细胞表达毛雷尔裂驻留蛋白，膜相关富组氨酸蛋白1（MAHRP 1）的绿色荧光蛋白（GFP）嵌合体。全长MAHRP 1的嵌合体或包含部分N-末端结构域和跨膜结构域的片段被成功地递送到Maurer裂。其他碎片仍然被困在寄生虫体内。荧光光漂白和延时成像技术表明，MAHRP 1-GFP最初被贩运到孤立的子域中的寄生虫空泡膜，似乎代表新生毛雷尔的裂缝。这些数据表明，毛雷尔的裂缝芽从寄生虫空泡膜和红细胞胞质内扩散，然后在细胞周边居住。
Malaria parasites export proteins beyond their own plasma membrane to locations in the red blood cells in which they reside. Maurer's clefts are parasite-derived structures within the host cell cytoplasm that are thought to function as a sorting compartment between the parasite and the erythrocyte membrane. However, the genesis of this compartment and the signals directing proteins to the Maurer's clefts are not known. We have generated Plasmodium falciparum-infected erythrocytes expressing green fluorescent protein (GFP) chimeras of a Maurer's cleft resident protein, the membrane-associated histidine-rich protein 1 (MAHRP1). Chimeras of full-length MAHRP1 or fragments containing part of the N-terminal domain and the transmembrane domain are successfully delivered to Maurer's clefts. Other fragments remain trapped within the parasite. Fluorescence photobleaching and time-lapse imaging techniques indicate that MAHRP1-GFP is initially trafficked to isolated subdomains in the parasitophorous vacuole membrane that appear to represent nascent Maurer's clefts. The data suggest that the Maurer's clefts bud from the parasitophorous vacuole membrane and diffuse within the erythrocyte cytoplasm before taking up residence at the cell periphery.