The effects of L-type amino acid transporter 1 on milk protein synthesis in mammary glands of dairy cows
The effects of L-type amino acid transporter 1 on milk protein synthesis in mammary glands of dairy cows
复制标题
L型氨基酸转运蛋白1对奶牛乳腺乳蛋白合成的影响。
DOI：
10.3168/jds.2017-13201
复制
发表时间：
2018-02-01
影响因子：
3.5
通讯作者：
Hou, Xiaoming
中科院分区：
文献类型：
--
作者：
Lin, Ye;Duan, Xiaoyu;Hou, Xiaoming
The mammary gland requires the uptake of AA for milk protein synthesis during lactation. The L-type amino acid transporter 1 (LAT1, encoded by SLC7A5), found in many different types of mammalian cells, is indispensable as a transporter of essential AA to maintain cell growth and protein synthesis. However, the function of LAT1 in regulating milk protein synthesis in the mammary gland of the dairy cow remains largely unknown. For the current study, we characterized the relationship between LAT1 expression and milk protein synthesis in lactating dairy cows and investigated whether the mammalian target of rapamycin complex 1 (mTORC1) signaling controls the expression of LAT1 in their mammary glands. We found that LAT1 and the heavy chain of its chaperone, 4F2, were expressed in mammary tissues of lactating cows, with the expression levels of LAT1 and the 4F2 heavy chain being significantly greater in lactating mammary tissues with high-milk protein content (milk yield, 33.8 +/- 2.1 kg/d; milk protein concentration >3%, wt/vol,; n = 3) than in tissues from cows with low-milk protein content (milk yield, 33.7 +/- 0.5 kg/d; milk protein concentration
