REPAIR OF CRITICAL SIZE RAT CALVARIAL DEFECTS USING EXTRACELLULAR-MATRIX PROTEIN GELS
REPAIR OF CRITICAL SIZE RAT CALVARIAL DEFECTS USING EXTRACELLULAR-MATRIX PROTEIN GELS
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DOI：
10.3171/jns.1995.83.4.0710
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发表时间：
1995-10-01
影响因子：
4.1
通讯作者：
OGLE, RC
中科院分区：
文献类型：
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作者：
SWEENEY, TM;OPPERMAN, LA;OGLE, RC
在这项研究中，作者研究了重建基底膜，层粘连蛋白和I型胶原蛋白的凝胶介导大鼠颅骨临界尺寸缺损修复的能力。尽管自体移植物被广泛用于修复由创伤或先天性畸形、肿瘤和感染的手术治疗引起的骨缺损，但并不总是有足够量的移植物可用。同种异体骨很容易获得，但其使用与骨不连、疲劳骨折和排斥反应的发生率增加有关。生物活性，纯化的成分的基底膜，这已被证明是促进成骨分化和血管生成在体外和I型胶原蛋白（骨细胞外基质的主要成分）可以形成到本地等渗的空间填充gels.In这项研究中的临界尺寸颅骨缺损创建退休的雄性Sprague-Dawley大鼠。将36只动物分为7组。第1组（对照组）未接受缺损治疗。第2组动物植入甲基纤维素。第3、4、5和6组分别植入I型胶原凝胶、重建基底膜或层粘连蛋白。最后一组三只动物（第7组）植入100 μ g I型胶原凝胶（与第3组相同），并在单次CT扫描后20周处死，以确定在足够的时间内用该方法是否可以获得完全愈合。除了I型胶原组的大鼠从2周到12周每两周通过多次计算机断层扫描（CT）进行评价外，在12周时使用CT评价骨修复。使用CT三维重建对愈合进行量化。在每个实验组中进行最终CT扫描后，处死动物，并通过常规组织学评价组织样品。用I型胶原凝胶治疗的动物在12周时显示87.5%的缺损区域修复，在20周时显示92.5%的修复。将凝胶体积增加1.5倍可将完全修复加速至3个月。鼠重构基底膜和层粘连蛋白凝胶诱导修复，分别为55.5%和46.3%，在3个月。在未经治疗的对照动物中，3个月时显示缺陷区域修复了7%。组织学分析证实在部分和完全愈合的缺损中有新骨形成。生物工程天然胶原蛋白凝胶可能具有广泛的适用性，作为骨修复的替代骨
In this study the authors examined the capacity of gels of reconstituted basement membrane, laminin, and type I collagen to mediate repair of critical size defects in rat calvaria. Although autografts are widely used to repair bone defects caused by trauma or surgical treatment of congenital malformations, neoplasms, and infections, an adequate quantity of graft is not always available. Allogenic bone is readily available, but its use is associated with an increased incidence of nonunion, fatigue fracture, and rejection. Biologically active, purified components of basement membranes, which have been shown to promote osteogenic differentiation and angiogenesis in vitro and type I collagen (the major constituent of bone extracellular matrix) can be formed into native isotonic space-filling gels.In this study critical size calvarial defects were created in retired male Sprague-Dawley rats. Thirty-six animals were divided into seven groups. Group 1 (control) received no treatment for the defects. Group 2 animals were implanted with methylcellulose. Groups 3, 4, 5, and 6 were implanted with gels of type I collagen, reconstituted basement membrane, or laminin, respectively. The last group of three animals (Group 7) was implanted with 100 mu g of type I collagen gels (identical to Group 3) and sacrificed at 20 weeks following a single CT scan to determine if complete healing could be obtained with this method given sufficient time. Except for rats in the type I collagen group that was evaluated by multiple computerized tomography (CT) scans biweekly from 2 to 12 weeks, bone repair was evaluated using CT at 12 weeks. Healing was quantified using three-dimensional reconstruction of CT. Following the final CT scan in each experimental group, animals were sacrificed, and a sample of tissues was evaluated by conventional histology. Animals treated with type I collagen gels showed 87.5% repair of the area of the defects at 12 weeks and 92.5% repair by 20 weeks. Increasing the gel volume 1.5 x accelerated complete repair to 3 months. Murine-reconstituted basement membrane and laminin gels induced 55.5% and 46.3% repair, respectively, at 3 months. In untreated control animals 7% repair of the area of the defects showed at 3 months. Histological analysis confirmed new bone formation in partial and completely healed defects. Bioengineered native collagen gels may have wide applicability for bone repair as an alternative bone