Effect of Fibroblasts on Epithelial Regeneration on the Surface of a Bioengineered Trachea
Effect of Fibroblasts on Epithelial Regeneration on the Surface of a Bioengineered Trachea
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DOI：
10.1177/000348940811700112
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发表时间：
2008-01
期刊：
影响因子：
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通讯作者：
Yukio Nomoto;Ken Kobayashi;Y. Tada;I. Wada;Tatsuo Nakamura;K. Omori
中科院分区：
文献类型：
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作者：
Yukio Nomoto;Ken Kobayashi;Y. Tada;I. Wada;Tatsuo Nakamura;K. Omori
目的：本课题组将以聚丙烯为支架，胶原海绵为支架的人工气管应用于首例人体气管移植，取得了成功。本研究的目的是找到一种方法，以获得更快的再上皮化与成纤维细胞的气管假体。方法：从大鼠气管分离气管上皮细胞，将其悬浮于胶原凝胶中。将具有成纤维细胞的胶原凝胶层压在胶原海绵上。将这种“生物工程气管”移植到大鼠气管缺损处，并对移植物上的再生上皮进行组织学检查。结果如下：植入后7天，复层鳞状上皮几乎覆盖了凝胶的所有表面，凝胶中的一些植入成纤维细胞排列在上皮下方。植入14天后，柱状和立方纤毛上皮几乎覆盖了整个缺损表面，植入的成纤维细胞已消失。植入后14 d再生上皮细胞数较无成纤维细胞对照组多，差异有统计学意义。结论：结果表明，由胶原海绵和胶原凝胶组成的生物工程气管与气管成纤维细胞组成的移植物在体内促进了上皮细胞的分化和增殖。
Objectives: Our group applied a tracheal prosthesis, which was composed of polypropylene as the frame and collagenous sponge as the scaffold, to the first human case and had successful results. The objective of this study was to find a way to acquire more rapid re-epithelialization with fibroblasts on this tracheal prosthesis. Methods: Tracheal epithelial cells, which were isolated from the trachea of rats, were suspended in a collagenous gel. The collagenous gel with fibroblasts was layered on a collagenous sponge. The grafts of this “bioengineered trachea” were implanted into tracheal defects of rats, and the regenerated epithelium on the grafts was histologically examined. Results: Seven days after implantation, stratified squamous epithelium covered almost all of the surface of the gel, and some of the implanted fibroblasts in the gel were lined up just below the epithelium. Fourteen days after implantation, columnar and cuboidal ciliated epithelium covered almost all of the surface of the defects, and the implanted fibroblasts had disappeared. The numbers of regenerated epithelial cells at 14 days after implantation were larger than those of control models without fibroblasts, with statistical significance. Conclusions: The results suggested that the grafts of bioengineered trachea composed of collagenous sponge and collagenous gel with tracheal fibroblasts accelerated epithelial differentiation and proliferation in vivo.