Effect of photodynamic therapy with hypocrellin B on apoptosis, adhesion, and migration of cancer cells
Effect of photodynamic therapy with hypocrellin B on apoptosis, adhesion, and migration of cancer cells
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DOI：
10.3109/09553002.2014.906765
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发表时间：
2014-07-01
影响因子：
2.6
通讯作者：
Xu, Chuanshan
中科院分区：
文献类型：
--
作者：
Jiang, Yuan;Leung, Albert Wingnang;Xu, Chuanshan
目的：在体外实验中，研究光动力疗法对肿瘤细胞凋亡、粘附和迁移的影响。材料与方法：将人卵巢癌HO-8910细胞作为肿瘤模型细胞，用2.5 μ m m浓度的hypocrellin B孵育5 h，用发光二极管光源照射。用膜联蛋白V/碘化丙啶（PI）染色和核染色的流式细胞术分析hypocrellin B光照射6 h后细胞凋亡情况。光动力处理4小时后，采用3-（4,5 -二甲基噻唑-2-基）- 2,5二苯基溴化四唑（MTT）测定法评估细胞粘附性。光动力处理48 h后测定细胞迁移量。结果：膜联蛋白V/PI染色流式细胞术显示，hypocrellin B光动力治疗后的早期凋亡和晚期凋亡（坏死）率分别显著升高至16.40%和24.67%。核染色发现处理后的细胞有核凝聚和典型的凋亡小体。经hypocrellin B光动力治疗后，细胞迁移数明显减少，为183 +/- 28 （p < 0.01）。单独光照射和单用hypocrellin B对细胞迁移无明显影响。光动力作用下的细胞粘附抑制率为53.2 +/- 1.8%，显著高于单光处理的2.7 +/- 2.1%和单光处理的1.0 +/- 0.4% （p < 0.01）。结论：hypocrellin B光动力治疗能显著诱导体外肿瘤细胞凋亡，抑制肿瘤细胞粘附和迁移。
Purpose: In the present study, we investigated effects of photodynamic therapy with hypocrellin B on apoptosis, adhesion, and migration of cancer cells in vitro.Materials and methods: Human ovarian cancer HO-8910 cell as a cancer model cell was incubated with hypocrellin B at a concentration of 2.5 mu m M for 5 h and irradiated by light from a light-emitting diodes (LED) source. Cell apoptosis was analyzed by flow cytometry with annexin V/propidium iodide (PI) staining and nuclear staining 6 h after hypocrellin B photoirradiation. Cell adhesion was assessed using the 3-(4, 5-dimthylthiazol-2-yl)-2, 5 diphenyl-tetrazolium bromide (MTT) assay 4 h after photodynamic treatment. Cell migration was measured 48 h after photodynamic treatment.Results: Flow cytometry with annexin V/PI staining showed that early apoptotic and late apoptotic (necrotic) rates following photodynamic therapy with hypocrellin B markedly increased to 16.40% and 24.67%, respectively. Nuclear staining found nuclear condensation and typical apoptotic body in the treated cells. The number of cell migration was significantly decreased to 183 +/- 28 after photodynamic therapy with hypocrellin B (p < 0.01). Light irradiation alone and hypocrellin B alone had no significant effect on cell migration. The cell adhesion inhibitory rate due to photodynamic action of hypocrellin B was 53.2 +/- 1.8%, significantly higher than 2.7 +/- 2.1% of light treatment alone and 1.0 +/- 0.4% of hypocrellin B treatment alone (p < 0.01).Conclusion: The findings demonstrated that photodynamic therapy with hypocrellin B remarkably induced apoptosis and inhibited adhesion and migration of cancer cells in vitro.