HIGH-RATE OF RECOMBINATION AND DOUBLE CROSSOVERS IN THE MOUSE PSEUDOAUTOSOMAL REGION DURING MALE MEIOSIS
HIGH-RATE OF RECOMBINATION AND DOUBLE CROSSOVERS IN THE MOUSE PSEUDOAUTOSOMAL REGION DURING MALE MEIOSIS
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DOI：
10.1073/pnas.84.20.7218
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发表时间：
1987-10-01
影响因子：
11.1
通讯作者：
JAENISCH, R
中科院分区：
文献类型：
--
作者：
SORIANO, P;KEITGES, EA;JAENISCH, R
分析了小鼠X染色体和Y染色体在减数分裂中的重组率。在两个假常染色体等位基因，类固醇硫酸酯酶基因和MOV-15前病毒标记，杂合小鼠进行了交叉。先前已证明Mov-15转基因小鼠品系中的前病毒携带在性染色体的假常染色体区域中。在雄性减数分裂中，该区域的染色体畸变频率是雌性减数分裂的7倍。三点杂交表明，在男性减数分裂的双重重组事件的发生在假常染色体区域，很少或没有交叉干扰，这是显着的对比观察人类性染色体的类似区域。这一结果与先前的模型相反，该模型预测了哺乳动物性染色体的雄性减数分裂配对中的单个交叉事件。
The recombination rate in meiosis between the mouse X and Y chromosomes was analyzed. Mice heterozygous at two pseudoautosomal alleles, the steroid sulfatase gene and the Mov-15 provirus marker, were crossed. The provirus in the Mov-15 transgenic mouse strain had been previously shown to be carried in the pseudoautosomal region of the sex chromosome. Recombination frequencies were shown to be 7-fold higher in this region in male meiosis than in female meiosis. Three-point crosses indicated the occurrence in male meiosis of double recombination events in the pseudoautosomal region, with little or no crossover interfernce, which is marked contrast to observations made on the similar region of the human sex chromsomes. This result is contrary to a previous model, which predicted a single crossover event in male meiotic pairing of mammalian sex chromosomes.