Papilloma formation by cottontail rabbit papillomavirus requires E1 and E2 regulatory genes in addition to E6 and E7 transforming genes.
Papilloma formation by cottontail rabbit papillomavirus requires E1 and E2 regulatory genes in addition to E6 and E7 transforming genes.
复制标题
棉尾兔乳头瘤病毒的乳头状瘤形成除了需要E6和E7转化基因外还需要E1和E2调节基因。
DOI：
10.1128/jvi.68.9.6097-6102.1994
复制
发表时间：
1994
影响因子：
5.4
通讯作者：
Brandsma,JL
中科院分区：
文献类型：
--
作者：
Wu,X;Xiao,W;Brandsma,JL
本研究利用棉尾兔乳头瘤病毒DNA-兔系统评价调控基因E1和E2以及转化基因E6是否在乳头状瘤的形成中起重要作用。在棉尾兔乳头瘤病毒全长基因组的背景下，在单个基因中产生移码突变，并将突变DNA皮内接种到家兔体内。突变体均未诱发乳头状瘤。标记挽救实验证实，缺陷是由于我们故意引入的突变造成的。标记物救援也证实了我们之前的报告，即E7上游第9位附近的区域对乳头状瘤的诱导至关重要。这些结果表明，在乳头状瘤的形成过程中，E1和E2调控基因以及E6和E7转化基因都是必需的。每个基因都可能为治疗干预提供分子靶点。
The present study used the cottontail rabbit papillomavirus DNA-rabbit system to evaluate whether the regulatory genes E1 and E2 and the transforming gene E6 are required for papilloma formation. Frameshift mutations were generated in the individual genes in the context of a full-length cottontail rabbit papillomavirus genome, and the mutant DNAs were intradermally inoculated into domestic rabbits. None of the mutants induced papillomas. Marker rescue experiments confirmed that the defects were due to mutations that we deliberately introduced. Marker rescue also confirmed our previous report that the upstream region of E7 around position 9 was critical for papilloma induction. These results demonstrate that the E1 and E2 regulatory genes as well as the E6 and E7 transforming genes are each required for papilloma formation. Each gene may provide molecular targets for therapeutic intervention.