Morphine and codeine from mammalian brain.
Morphine and codeine from mammalian brain.
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来自哺乳动物大脑的吗啡和可待因。
DOI：
10.1073/pnas.83.24.9784
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发表时间：
1986
影响因子：
11.1
通讯作者：
Goldstein,A
中科院分区：
文献类型：
--
作者：
Weitz,CJ;Lowney,LI;Faull,KF;Feistner,G;Goldstein,A
最近，我们描述了牛肾上腺和下丘脑中存在六种免疫反应性 (ir) 吗啡喃，并鉴定其中一种为吗啡 [Goldstein, A.、Barrett, R. W.、James, I. F.、Lowney, L. I.、Weitz, C. J.、Knipmeyer, L. L. 和 Rapaport, H. (1985) Proc.国家。阿卡德。科学。美国 82, 5203-5207]。我们现在报道，对应于先前报道的峰 1（吗啡）、峰 4 和峰 5 的 ir 吗啡喃始终存在于牛下丘脑提取物中，并且不同程度地存在于牛肾上腺和大鼠脑提取物中。我们不再检测到先前报告的峰 2、3 或 6，并且我们已确定它们是污染伪影。峰 1 在两个不同的反相 HPLC 系统中与吗啡共洗脱，峰 4 与可待因也是如此。我们从牛下丘脑纯化了峰 1 和峰 4 化合物，并通过气相色谱/质谱 (GC/MS) 确定了它们的身份：确认峰 1 是吗啡，峰 4 是可待因。
Recently, we described the presence of six immunoreactive (ir) morphinans in bovine adrenal and hypothalamus and identified one as morphine [Goldstein, A., Barrett, R. W., James, I. F., Lowney, L. I., Weitz, C. J., Knipmeyer, L. L. & Rapaport, H. (1985) Proc. Natl. Acad. Sci. USA 82, 5203-5207]. We now report that ir morphinans corresponding to the previously reported peak 1 (morphine), peak 4, and peak 5 are consistently present in extracts of bovine hypothalamus and variably present in extracts of bovine adrenal and rat brain. We no longer detect the previously reported peaks 2, 3, or 6, and we have established that they were contamination artifacts. Peak 1 is coeluted with morphine in two distinct reversed-phase HPLC systems, as is peak 4 with codeine. We have purified peak 1 and peak 4 compounds from bovine hypothalamus and determined their identities by gas chromatography/mass spectrometry (GC/MS): peak 1 is confirmed to be morphine and peak 4 is codeine.