Three-dimensional polycaprolactone-hydroxyapatite scaffolds combined with bone marrow cells for cartilage tissue engineering
Three-dimensional polycaprolactone-hydroxyapatite scaffolds combined with bone marrow cells for cartilage tissue engineering
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DOI：
10.1177/0885328215575762
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发表时间：
2015-08-01
影响因子：
2.9
通讯作者：
Wang, Liming
中科院分区：
文献类型：
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作者：
Wei, Bo;Yao, Qingqiang;Wang, Liming
本研究的目的是探讨三维聚己内酯-羟基磷灰石（PCL-HA）支架体外负载骨髓细胞的成软骨潜能及其对骨软骨缺损修复的影响。在此，将骨髓加入到制备的PCL-HA支架中，并在软骨形成培养基中培养10周。在17只新西兰白色兔的29个膝关节的股骨槽中产生骨软骨缺损，然后将其分为四组，进行：植入PCL-HA支架（左膝，n=17;组1），微骨折（右膝，n=6;组2），自体骨软骨移植（右膝，n=6;组3），和不治疗（右膝，n=5;对照）。培养10周后，骨髓细胞产生的细胞外基质覆盖在PCL-HA支架的表面并填充其孔隙。此外，观察到许多载有细胞的软骨陷窝，软骨基质集中在PCL-HA支架中。经过12周的修复期，第1组显示出良好的垂直和横向与宿主骨的整合，但不完全的软骨再生和基质积累。在第2组中观察到再生软骨表面不均匀和软骨基质分布减少。此外，检测到异常的骨生长和修复组织与宿主组织之间的不稳定整合。对于第3组，移植软骨与宿主软骨之间的整合中断。我们的研究结果表明，负载骨髓细胞的PCL-HA支架促进了体外软骨形成，并且用于骨软骨修复的PCL-HA支架植入促进了与宿主骨的整合。然而，软骨再生仍然不令人满意。添加营养因子或使用预培养细胞-PCL-HA结构加速骨软骨修复需要进一步研究。
The goal of this study was to investigate the chondrogenic potential of three-dimensional polycaprolactone-hydroxyapatite (PCL-HA) scaffolds loaded with bone marrow cells invitro and the effect of PCL-HA scaffolds on osteochondral repair invivo. Here, bone marrow was added to the prepared PCL-HA scaffolds and cultured in chondrogenic medium for 10 weeks. Osteochondral defects were created in the trochlear groove of 29 knees in 17 New Zealand white rabbits, which were then divided into four groups that underwent: implantation of PCL-HA scaffolds (left knee, n=17; Group 1), microfracture (right knee, n=6; Group 2), autologous osteochondral transplantation (right knee, n=6; Group 3), and no treatment (right knee, n=5; Control). Extracellular matrix produced by bone marrow cells covered the surface and filled the pores of PCL-HA scaffolds after 10 weeks in culture. Moreover, many cell-laden cartilage lacunae were observed, and cartilage matrix was concentrated in the PCL-HA scaffolds. After a 12-week repair period, Group 1 showed excellent vertical and lateral integration with host bone, but incomplete cartilage regeneration and matrix accumulation. An uneven surface of regenerated cartilage and reduced distribution of cartilage matrix were observed in Group 2. In addition, abnormal bone growth and unstable integration between repaired and host tissues were detected. For Group 3, the integration between transplanted and host cartilage was interrupted. Our findings indicate that the PCL-HA scaffolds loaded with bone marrow cells improved chondrogenesis invitro and implantation of PCL-HA scaffolds for osteochondral repairenhanced integration with host bone. However, cartilage regeneration remained unsatisfactory. The addition of trophic factors or the use of precultured cell-PCL-HA constructs for accelerated osteochondral repair requires further investigation.