Nuclear magnetic resonance spectroscopy-based metabolite profiling of transgenic tomato fruit engineered to accumulate spermidine and spermine reveals enhanced anabolic and nitrogen-carbon interactions
Nuclear magnetic resonance spectroscopy-based metabolite profiling of transgenic tomato fruit engineered to accumulate spermidine and spermine reveals enhanced anabolic and nitrogen-carbon interactions
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DOI：
10.1104/pp.106.084400
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发表时间：
2006-12-01
期刊：
影响因子：
7.4
通讯作者：
Segre, Anna L.
中科院分区：
文献类型：
--
作者：
Mattoo, Autar K.;Sobolev, Anatoli P.;Segre, Anna L.
多胺是普遍存在的脂肪族胺，涉及无数的过程，但其确切的生物化学作用尚未完全了解。我们已经进行了转基因番茄（Solanum lycopersicum）果实的代谢产物谱分析，工程积累更高的多胺亚精胺（Spd）和精胺（Spm），使深入了解Spd/Spm调节植物的代谢过程。NMR光谱分析揭示了转基因和野生型/azygous成熟的葡萄果实中不同的代谢物趋势。不同的代谢产物（谷氨酰胺，天冬酰胺，胆碱，柠檬酸，富马酸，苹果酸，和一个身份不明的化合物A）积累在红色的转基因水果，而缬氨酸，天冬氨酸，蔗糖和葡萄糖的水平显着较低的控制（野生型和azygous）红色水果。异亮氨酸，葡萄糖，γ-氨基丁酸，苯丙氨酸和果糖的水平在非转基因和转基因水果中保持相似。代谢物变量的统计学处理将对照果实与转基因果实区分开来，并为转基因果实成熟期间观察到的显著差异代谢物谱提供了证据。参与氮传感/信号传导和碳代谢的途径似乎在高Spd/Spm转基因中优先激活。代谢产物谱分析表明，Spd和Spm被果实细胞视为含氮代谢产物，这反过来又导致了对固碳的刺激。与对照相比，这表现在转基因果实中较高的呼吸活性和磷酸烯醇式丙酮酸羧化酶和NADP依赖性异柠檬酸脱氢酶转录物的上调，表明转基因果实甚至在果实成熟后期的高代谢状态。
Polyamines are ubiquitous aliphatic amines that have been implicated in myriad processes, but their precise biochemical roles are not fully understood. We have carried out metabolite profiling analyses of transgenic tomato (Solanum lycopersicum) fruit engineered to accumulate the higher polyamines spermidine (Spd) and spermine (Spm) to bring an insight into the metabolic processes that Spd/Spm regulate in plants. NMR spectroscopic analysis revealed distinct metabolite trends in the transgenic and wild-type/azygous fruits ripened off the vine. Distinct metabolites (glutamine, asparagine, choline, citrate, fumarate, malate, and an unidentified compound A) accumulated in the red transgenic fruit, while the levels of valine, aspartic acid, sucrose, and glucose were significantly lower as compared to the control (wild-type and azygous) red fruit. The levels of isoleucine, glucose, gamma-aminobutyrate, phenylalanine, and fructose remained similar in the nontransgenic and transgenic fruits. Statistical treatment of the metabolite variables distinguished the control fruits from the transgenic fruit and provided credence to the pronounced, differential metabolite profiles seen during ripening of the transgenic fruits. The pathways involved in the nitrogen sensing/signaling and carbon metabolism seem preferentially activated in the high Spd/Spm transgenics. The metabolite profiling analysis suggests that Spd and Spm are perceived as nitrogenous metabolites by the fruit cells, which in turn results in the stimulation of carbon sequestration. This is seen manifested in higher respiratory activity and up-regulation of phosphoenolpyruvate carboxylase and NADP-dependent isocitrate dehydrogenase transcripts in the transgenic fruit compared to controls, indicating high metabolic status of the transgenics even late in fruit ripening.