A three-dimensional digital atlas database of the adult C57BL/6J mouse brain by magnetic resonance microscopy
A three-dimensional digital atlas database of the adult C57BL/6J mouse brain by magnetic resonance microscopy
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DOI：
10.1016/j.neuroscience.2005.07.014
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发表时间：
2005-01-01
期刊：
影响因子：
3.3
通讯作者：
Benveniste, H
中科院分区：
文献类型：
--
作者：
Ma, Y;Hof, PR;Benveniste, H
一个全面的三维数字图谱数据库的C57 BL/6 J小鼠大脑的基础上获得的17.6 T超导磁体的磁共振显微镜图像。通过使用手动跟踪和基于图谱的半自动分割方法，T2* 加权磁共振显微镜图像的10个成年雄性福尔马林固定，切除C57 BL/6 J小鼠大脑被分割成20个解剖结构。这些结构包括新皮质、海马、杏仁核、嗅球、基底前脑和隔膜、尾壳核、苍白球、丘脑、下丘脑、中央灰质、上级丘、下丘、中脑的其余部分、小脑、脑干、胼胝体/外囊、内囊、前连合、海马伞和脑室。将分割数据格式化并存储到包含三种不同图谱类型的数据库中：10个单标本脑图谱，平均脑图谱和概率图谱。此外，定量组信息，如结构体积，表面积，磁共振显微镜图像强度和局部几何形状的变化，计算和存储作为数据库的一个组成部分。该数据库与小鼠表型组数据库所定义的其他高优先级菌株一起增强了正在进行的努力，该数据库专注于为通过无数技术和成像模式获得的功能，遗传和蛋白质表达模式的准确映射提供定量框架。(c)2005年IBRO。由爱思唯尔有限公司出版。保留所有权利。
A comprehensive three-dimensional digital atlas database of the C57BL/6J mouse brain was developed based on magnetic resonance microscopy images acquired on a 17.6-T superconducting magnet. By using both manual tracing and an atlas-based semi-automatic segmentation approach, T2*-weighted magnetic resonance microscopy images of 10 adult male formalin-fixed, excised C57BL/6J mouse brains were segmented into 20 anatomical structures. These structures included the neocortex, hippocampus, amygdala, olfactory bulbs, basal forebrain and septum, caudate-putamen, globus pallidus, thalamus, hypothalamus, central gray, superior colliculi, inferior colliculi, the rest of midbrain, cerebellum, brainstem, corpus callosum/external capsule, internal capsule, anterior commissure, fimbria, and ventricles. The segmentation data were formatted and stored into a database containing three different atlas types: 10 single-specimen brain atlases, an average brain atlas and a probabilistic atlas. Additionally, quantitative group information, such as variations in structural volume, surface area, magnetic resonance microscopy image intensity and local geometry, were computed and stored as an integral part of the database. The database augments ongoing efforts with other high priority strains as defined by the Mouse Phenome Database focused on providing a quantitative framework for accurate mapping of functional, genetic and protein expression patterns acquired by a myriad of technologies and imaging modalities. (c) 2005 IBRO. Published by Elsevier Ltd. All rights reserved.