Biomarkers of oxidation stress, inflammation, necrosis and apoptosis are associated with hepatitis B-related acute-on-chronic liver failure
Biomarkers of oxidation stress, inflammation, necrosis and apoptosis are associated with hepatitis B-related acute-on-chronic liver failure
复制标题
DOI：
10.1016/j.clinre.2015.06.009
复制
发表时间：
2016-02-01
影响因子：
2.7
通讯作者：
Gao, Yingtang
中科院分区：
文献类型：
--
作者：
Cai, Junjun;Han, Tao;Gao, Yingtang
背景与目的：B型肝炎病毒相关的慢加急性肝衰竭是一种严重的疾病，病死率高。本研究旨在探讨血清AOPP、S100 A12、HMGB 1和sIgA在HBV相关ACLF中的诊断和预后价值。我们检测了50例HBV相关ACLF患者、35例肝硬化（LC）患者、30例正常对照组和30例正常对照组血清S100 A12、HMGB 1和sIgA水平，应用酶联免疫吸附试验检测35例慢性B型肝炎（CH B）患者和35例健康对照者血清中HBV DNA的含量。结果：ACLF患者入院时AOPP、S100 A12、HMGB 1和sIgA水平均显著高于LC、CHB和健康对照组（P < 0.001）。ACLF患者中死亡者高于存活者（P < 0.001）。与总胆红素、MELD评分呈正相关。AOPP、S100 A12和HMGB 1浓度在存活者中持续下降，而在死亡者中持续升高，血清AOPP浓度在存活者中无变化，而在死亡者中逐渐升高。ROC曲线分析显示，4种生物标志物的AUC均高于TBIL。多因素考克斯回归分析显示，血清S100 A12、AOPP和sHBV-DNA是影响预后的独立危险因素。结论：血清AOPP、S100 A12和sHBV-DNA可能反映了HBV相关慢加急性肝衰竭患者氧化应激、炎症反应水平。AOPP、S100 A12和sCRP升高可能是预后不良的重要生物学标志物。(C)2015年Elsevier Masson SAS。All rights reserved.
Background and objective: Hepatitis B virus related acute-on-chronic liver failure is a serious condition with a high mortality. Oxidative stress, inflammation, necrosis and apoptosis may play an important role in it. This study is to investigate whether serum AOPP, S100A12, HMGB1 and sRAGE can provide diagnostic or prognostic information in HBV-related ACLF.Methods: We measured serum S100A12, HMGB1 and sRAGE levels in 50 patients with HBV-related ACLF, 35 patients with liver cirrhosis (LC), 35 patients with chronic hepatitis B (CHB) and 35 healthy controls by enzyme-linked immunosorbent assay. AOPP measured by spectrophotometry.Results: Significantly higher AOPP, S100A12, HMGB1 and sRAGE levels on admission were found in patients with ACLF compared with LC, CHB and healthy controls (P < 0.001). In ACLF patients, they were higher in nonsurvivors than survivors (P < 0.001). They had a positive relationship with total bilirubin and MELD scores. AOPP, S100A12 and HMGB1 concentrations continually declined in survivors while increased in nonsurvivors, sRAGE concentrations did not change in survivors, but gradually increased in nonsurvivors during hospitalization. ROC curve analysis showed that the four biomarkers had a higher AUC than TBIL. Multivariate Cox regression analysis demonstrated that S100A12, AOPP and sRAGE were independent risk factors for poor prognosis.Conclusion: Serum AOPP, S100A12 and sRAGE maybe reflect the oxidation stress, inflammation levels in HBV-related acute-on-chronic liver failure. Increased AOPP, S100A12 and sRAGE may serve as important biological markers of worse outcome. (C) 2015 Elsevier Masson SAS. All rights reserved.