Comparison between ImmunoCAP and multiple antigen simultaneous tests for measuring Aspergillus -specific Immunoglobulin E levels in Aspergillus -sensitized patients
Comparison between ImmunoCAP and multiple antigen simultaneous tests for measuring Aspergillus -specific Immunoglobulin E levels in Aspergillus -sensitized patients
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比较ImmunoCAP和多抗原同时检测在测量曲霉菌致敏患者中曲霉菌特异性免疫球蛋白E水平
DOI：
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
T. Horiguchi
中科院分区：
文献类型：
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作者：
K. Kuwabara;Tatsuyoshi Yokoi;Takazumi Yoshida;M. Shiga;M. Hirose;R. Kondo;K. Matsunaga;M. Nakamura;T. Horiguchi
目的：曲霉致敏对哮喘患者有重要意义。在日本，用于测量血液中过敏原特异性免疫球蛋白E （IgE）水平的方法是单抗原测试，免疫cap （IC）和多抗原同时测试，View Allergy®（VA）或MAST IV®（MA）。在这里，我们报告了曲霉特异性IgE水平在IC和VA或MA之间的一致性率（CR）。方法：采用ImmunoCAP、View Allergy®（Thermo Fisher Scientific, Uppsala, Sweden）和MAST IV®（Hitachi Chemical Diagnostics, Inc.）检测34例男性和23例女性支气管哮喘患者血清中曲霉菌特异性IgE水平。山景城，加州，美国)。以1级及以上的结果为阳性，并评估两种方法间的cr。结果：57例患者中，IC检测曲霉特异性IgE阳性24例，变应性支气管肺曲霉病（ABPA） 5例。IC与VA （r=0.964, p <0.001）、IC与MA （r=0.620, p <0.001）具有显著的类内相关性。IC与VA之间的CR为98.2%，阳性一致性率为100%，阴性一致性率为96.9%；在IC和MA之间，这些值分别为77.2%，45.8%和100%。所有5例ABPA患者均经VA检测呈曲霉特异性IgE阳性，而其中只有3例（60%）经MA检测呈阳性。结论：在哮喘患者中，与MA相比，IC测量结果与VA测量结果更为一致。
Objectives: Aspergillus sensitization is important for patients with asthma. In Japan, the methods applied to measure allergen-specific immunoglobulin E (IgE) levels in blood are the single antigen test, ImmunoCAP (IC), and multiple antigen simultaneous tests, View Allergy ® (VA) or MAST IV ® (MA). Here, we report the concordance rates (CR) for Aspergillus -specific IgE levels between IC and VA or MA. Methods: Aspergillus -specific IgE levels in serum samples from 34 male and 23 female patients with bronchial asthma were measured by ImmunoCAP, View Allergy ® (both Thermo Fisher Scientific, Uppsala, Sweden) and MAST IV ® (Hitachi Chemical Diagnostics, Inc. Mountain View, CA, USA). Results of Class 1 or greater were regarded as positive, and the CRs between the methods were assessed. Results: Of the 57 patients, 24 were found to be positive for Aspergillus -specific IgE by IC, and 5 had allergic bronchopulmonary aspergillosis (ABPA). Significant intraclass correlations were observed between IC and VA (r=0.964, p <0.001) and between IC and MA (r=0.620, p <0.001). Between IC and VA, the CR, positive concordance ratio, and negative concordance ratio was 98.2%, 100%, and 96.9%, respectively; between IC and MA, these values were 77.2%, 45.8%, and 100%, respectively. All five patients with ABPA were found to be positive for Aspergillus specific IgE by VA, whereas only three of these patients (60%) were found to be positive by MA. Conclusions: In patients with asthma, measurements obtained by IC were more concordant with those obtained by VA compared with those obtained by MA.
有或没有支气管扩张证据的患者的过敏性支气管肺曲霉病。
DOI：
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发表时间：
1993
期刊：
Annals of allergy
影响因子：
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作者：
Greenberger,PA;Miller,TP;Roberts,M;Smith,LL
通讯作者：
Smith,LL