Prediction of the location of stationary steady-state zone positions in counterflow isotachophoresis performed under constant voltage in a vortex-stabilized annular column
Prediction of the location of stationary steady-state zone positions in counterflow isotachophoresis performed under constant voltage in a vortex-stabilized annular column
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DOI：
10.1002/jssc.200700243
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发表时间：
2007-12-01
影响因子：
3.1
通讯作者：
Ivory, Cornelius F.
中科院分区：
文献类型：
--
作者：
Harrison, Schurie L. M.;Ivory, Cornelius F.
提出了一个理论模型，并推导出一个解析表达式来预测ITP中的固定稳态区位置作为电流的函数，在恒定的施加电压下的直通道。静止区可在逆流存在下形成，逆流的平均速度福尔斯落在纯前导区和纯拖尾区之间。从阴离子系统的实验数据与模型预测的比较表明，该模型是能够预测的蛋白质区的位置与合理的准确性，一旦ITP堆栈已经形成。该结果意味着ITP堆叠可以由操作者精确地引导到通道中的特定位置，在该通道中堆叠的部分可以被移除或重新定向以用于进一步处理或分析。
A theoretical model is presented and an analytical expression derived to predict the locations of stationary steady-state zone positions in ITP as a function of current for a straight channel under a constant applied voltage. Stationary zones may form in the presence of a countercurrent flow whose average velocity falls between that of a pure leader zone and of a pure trailer zone. A comparison of model predictions with experimental data from an anionic system shows that the model is able to predict the location of protein zones with reasonable accuracy once the ITP stack has formed. This result implies that an ITP stack can be precisely directed by the operator to specific positions in a channel whence portions of the stack can be removed or redirected for further processing or analysis.