BOTH ACTIVATION AND REPRESSION OF A-MATING-TYPE-SPECIFIC GENES IN YEAST REQUIRE TRANSCRIPTION FACTOR MCM1
BOTH ACTIVATION AND REPRESSION OF A-MATING-TYPE-SPECIFIC GENES IN YEAST REQUIRE TRANSCRIPTION FACTOR MCM1
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DOI：
10.1073/pnas.88.23.10966
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发表时间：
1991-12-01
影响因子：
11.1
通讯作者：
TYE, BK
中科院分区：
文献类型：
--
作者：
ELBLE, R;TYE, BK
Mcm1是一种酵母转录因子，在后生动物中具有同源物。MCM1基因首次被鉴定为与酵母中人工微染色体维持有关的基因。最近，Mcm1被证明是交配型特定基因的转录调节器。生化数据表明，Mcm1通过与上游区域的10个碱基对的二联体对称元件结合，共同激活α-特异基因，并核心抑制α-特异基因。我们之前曾报道，mcm1点突变降低了阿尔法特异基因的激活，但对α特异基因的表达几乎没有影响。我们现在证明了另一个mcm1等位基因，它耗尽了mcm1蛋白，影响a-特异性基因的激活和抑制。突变菌株仍然能够高水平地诱导a-特异性基因的信息素诱导，尽管动力学迟缓。Mcm1加入了越来越多的转录因子，参与了基因表达的正向和负向调节。
Mcm1 is a yeast transcription factor with homologs throughout the metazoa. MCM1 was first identified as a gene involved in maintenance of artificial minichromosomes in yeast. More recently Mcm1 has been shown to serve as a transcriptional regulator of mating-type-specific genes. Biochemical data suggest that Mcm1 coactivates alpha-specific genes and corepresses a-specific genes by binding to a 10-base-pair dyad symmetry element in their upstream regions. We reported previously that an mcm1 point mutation reduced activation of alpha-specific genes but had little effect on the expression of a-specific genes. We now show that another mcm1 allele, which depletes the Mcm1 protein, affects both activation and repression of a-specific genes. The mutant strain remains capable of high levels of pheromone induction of a-specific genes, although with retarded kinetics. Mcm1 joins an increasing number of transcription factors involved in both positive and negative regulation of gene expression.