Self-assembly properties and dynamic of synthetic proteo-nucleic building blocks in solution and on surfaces
Self-assembly properties and dynamic of synthetic proteo-nucleic building blocks in solution and on surfaces
复制标题
溶液中和表面上合成蛋白核构建块的自组装特性和动态
DOI：
10.1021/bc2002264
复制
发表时间：
2011
影响因子：
4.7
通讯作者：
D.Pompon
中科院分区：
文献类型：
--
作者：
A.Laisne;M.Ewald;T.Ando;E.Lesniewska;D.Pompon
支气管哮喘患者呼出气一氧化氮（ENO）水平升高，吸入糖皮质激素治疗可降低ENO水平。ENO似乎是一种炎症标志物，但其在咳嗽病理生理学中的作用尚不清楚。本研究旨在阐明NO与气道变态反应引起的咳嗽反射敏感性增加的关系。方法在未致敏和卵清蛋白（OVA）致敏豚鼠中，用NO合成酶（NOS）抑制剂引起NO耗竭，观察吸入辣椒素引起的咳嗽反射敏感性。结果用非选择性NOS抑制剂L-NAME抑制非致敏豚鼠的咳嗽反射敏感性和OVA诱导的致敏豚鼠咳嗽反射敏感性的增加;然而，由ONO 1714引起的iNOS抑制部分抑制了OVA诱导的咳嗽反射敏感性的增加，但不能抑制非致敏豚鼠的正常咳嗽反应。ONO 1714没有改变BAL细胞成分在OVA致敏的豚鼠。ConclusionsThe结果表明，NO可能不仅参与正常的咳嗽反射电路，但也在OVA诱导的咳嗽反射敏感性增加，可能通过不同的作用机制。需要进一步的研究来阐明确切的机制。
BackgroundExhaled nitric oxide (ENO) is elevated in bronchial asthma patients, and inhaled corticosteroid therapy lowers the elevated ENO levels in such patients. ENO appears to be an inflammatory marker, but its role in the pathophysiology of cough remains unclear. This study aimed to elucidate the relationship between NO and increased cough reflex sensitivity induced by allergic airway reactions.MethodsCough reflex sensitivity to inhaled capsaicin was observed under NO depletion caused by NO synthase (NOS) inhibitors in non-sensitized and ovalbumin (OVA)-sensitized guinea pigs. The bronchoalveolar lavage fluid (BALF) was analyzed in an NO depletion setting using the inducible NOS (iNOS) inhibitor ONO1714 in OVA-sensitized guinea pigs.ResultsNO depletion by the non-selective NOS inhibitor L-NAME suppressed cough reflex sensitivity in non-sensitized guinea pigs and OVA-induced increase in cough reflex sensitivity in sensitized guinea pigs; however, iNOS inhibition caused by ONO1714 partially suppressed the OVA-induced increase in cough reflex sensitivity, but not the normal cough response in non-sensitized guinea pigs. ONO1714 did not change BAL cell components in OVA-sensitized guinea pigs.ConclusionsThe results suggest that NO may be involved not only in the normal cough reflex circuit, but also in the OVA-induced increase in cough reflex sensitivity, possibly via a different mechanism of action. Further studies are needed to clarify the precise mechanism.