Differential expression of glycine receptor subunits in the rat basolateral and central amygdala
Differential expression of glycine receptor subunits in the rat basolateral and central amygdala
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DOI：
10.1016/j.neulet.2009.12.003
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发表时间：
2010-01-22
影响因子：
2.5
通讯作者：
Sah, P.
中科院分区：
文献类型：
--
作者：
Delaney, A. J.;Esmaeili, A.;Sah, P.
杏仁核复合体是一种边缘系统结构，在情绪处理和恐惧条件反射中起着关键作用。虽然杏仁核中的抑制性传递主要是GABA能的，但已知杏仁核的神经元也表达甘氨酸受体。杏仁核突触回路中这些甘氨酸受体的亚型和功能尚不清楚。在这项研究中，我们研究了四个主要的甘氨酸受体亚基（α 1-3和β）在大鼠基底外侧（BLA）和中央杏仁核（CeA）的相对表达，使用实时PCR和蛋白质生物化学。我们证明，α 1，α 2，α 3和β亚基都表达在BLA和CeA与α 2是主要的α亚基在两个核。急性脑切片中BLA和CeA神经元的电生理记录表明，这些亚基的相对表达的差异与这些神经元上表达的天然甘氨酸受体的药理学特性相关。我们的结论是，甘氨酸受体组装在BLA神经元主要是α 1 β-含异多聚体，而受体组装在中央杏仁核的神经元主要是α 2 β-，α 3 β-或α 1 β-含异多聚体，与α 2或α 3同源受体的次要成分也表达。(C)2009爱思唯尔爱尔兰有限公司保留所有权利。
The amygdalar complex is a limbic structure that plays a key role in emotional processing and fear conditioning. Although inhibitory transmission in the amygdala is predominately GABA-ergic, neurons of the amygdala are also known to express glycine receptors. The subtype and function of these glycine receptors within the synaptic circuits of the amygdala are unknown. In this study, we have investigated the relative expression of the four major glycine receptor subunits (alpha 1-3 and beta) in the rat basolateral (BLA) and central amygdala (CeA), using real-time PCR and protein biochemistry. We demonstrate that alpha 1, alpha 2, alpha 3, and beta subunits are all expressed in the BLA and CeA with alpha 2 being the predominant alpha-subunit in both nuclei. Electrophysiological recordings from BLA and CeA neurons in acute brain slices indicated that differences in relative expression of these subunits were correlated with the pharmacological properties of native glycine receptors expressed on these neurons. We conclude that glycine receptors assembled in BLA neurons are largely alpha 1 beta-containing heteromultimers whereas receptors assembled in neurons of the central amygdala are primarily alpha 2 beta-, alpha 3 beta- or alpha 1 beta-containing heteromultimers, with a minor component of alpha 2 or alpha 3 homomeric receptors also expressed. (C) 2009 Elsevier Ireland Ltd. All rights reserved.