Modified method of silver staining of proteins in polyacrylamide gels.
Modified method of silver staining of proteins in polyacrylamide gels.
复制标题
聚丙烯酰胺凝胶中蛋白质银染色的改进方法。
DOI：
10.1016/0003-2697(86)90349-0
复制
发表时间：
1986
影响因子：
2.9
通讯作者：
Hnilica,LS
中科院分区：
文献类型：
--
作者：
Wedrychowski,A;Olinski,R;Hnilica,LS
Wray等人（Anal.（1981）118，197-203）在蛋白质样品含有核酸和/或金属时失效。通过在乙酸中洗涤聚丙烯酰胺凝胶，并在电泳后立即在甲醇中反复洗涤，然后使用Wray等人的方法，许多其它未染色的凝胶可以高度可靠地染色。这种方法允许在含有大量DNA和金属的样品中观察微量蛋白质。
The very sensitive and reliable silver staining method to visualize proteins in polyacrylamide gels described by Wray et al. (Anal. Biochem. (1981) 118, 197–203) fails when the protein sample contains nucleic acids and/or metals. By washing the polyacrylamide gels in acetic acid and repeatedly in methanol immediately following electrophoresis and then using the procedure of Wray et al., many gels otherwise unstainable may be stained with a high degree of reliability. This method allows visualization of a minute amount of proteins in samples containing high amounts of DNA and metals.