Genetic and antigenic diversity among eastern equine encephalitis viruses from North, Central, and South America
Genetic and antigenic diversity among eastern equine encephalitis viruses from North, Central, and South America
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DOI：
10.4269/ajtmh.1999.61.579
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发表时间：
1999-10-01
影响因子：
3.3
通讯作者：
Weaver, SC
中科院分区：
文献类型：
--
作者：
Brault, AC;Powers, AM;Weaver, SC
东部马脑炎病毒（EEEV）是EEE抗原复合物中的唯一物种，根据血凝抑制试验将其分为北美和南美抗原变种。在这里，我们描述了这些品种的代表，跨越整个时间和地理范围内可用的血清学和系统发育分析。核苷酸测序和系统发育分析揭示了南美品种内的额外的遗传多样性;确定了3个主要的南美/中美洲谱系，包括一个由来自巴西东部的单一分离株代表的谱系，以及2个在中美洲和南美洲分布更广泛的谱系。所有北美分离株都包含一个单一的、高度保守的谱系，其中菌株按分离时间分组，并在一定程度上按位置分组。1996年在墨西哥塔毛利帕斯州马疫情期间分离的EEEV菌株与最近的德克萨斯州分离株密切相关，表明向南EEEV运输超出了假定的地方性流行范围。来自4个主要谱系的代表进行的空斑减少中和试验表明，每个谱系代表不同的抗原亚型。对该复合体的分类学进行了修订。
Eastern equine encephalitis virus (EEEV), the sole species in the EEE antigenic complex, is divided into North and South American antigenic varieties based on hemagglutination inhibition tests. Here we describe serologic and phylogenetic analyses of representatives of these varieties, spanning the entire temporal and geographic range available. Nucleotide sequencing and phylogenetic analyses revealed additional genetic diversity within the South American variety; 3 major South/Central American lineages were identified including one represented by a single isolate from eastern Brazil, and 2 lineages with more widespread distributions in Central and South America. All North American isolates comprised a single, highly conserved lineage with strains grouped by the time of isolation and to some extent by location. An EEEV strain isolated during a 1996 equine outbreak in Tamaulipas State, Mexico was closely related to recent Texas isolates, suggesting southward EEEV transportation beyond the presumed enzootic range. Plaque reduction neutralization tests with representatives from the 4 major lineages indicated that each represents a distinct antigenic subtype. A taxonomic revision pf the EEE complex is proposed.