Nucleotide sequence of the 5′-terminus of Newcastle disease virus and assembly of the complete genomic sequence:: agreement with the "rule of six"
Nucleotide sequence of the 5′-terminus of Newcastle disease virus and assembly of the complete genomic sequence:: agreement with the "rule of six"
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DOI：
10.1007/s007050050435
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发表时间：
1998-01-01
影响因子：
2.7
通讯作者：
Emmerson, PT
中科院分区：
文献类型：
--
作者：
Phillips, RJ;Samson, ACR;Emmerson, PT
我们已经确定了三株新城疫病毒的5′端序列，使整个基因组序列得以组装，总计15,186个核苷酸。这个长度与六法则一致，六法则已被证明可以决定类似病毒的复制效率。将该病毒尾部尾部5′端序列与3′端先导序列进行比较，发现两者具有高度的互补性。不同菌株5′端序列的差异表明存在不同的L基因多腺苷化信号，导致相应的拖车长度不同。
We have determined the sequences of the 5' ends of three strains of Newcastle disease virus, permitting the assembly of the entire genomic sequence, which amounts to 15,186 nucleotides. This length is in agreement with the rule of six, which has been shown to determine replication efficiency in similar viruses. Comparison of the extreme 5' end of the trailer sequence with that of the 3'-terminal leader sequence of the virus reveals a high degree of complementarity. Variation between the 5'-terminal sequences of the different strains reveals the presence of alternative L gene polyadenylation signals, leading to correspondingly different trailer lengths.