Adherence of Vibrio cholerae to cultured differentiated human intestinal cells: an in vitro colonization model.
Adherence of Vibrio cholerae to cultured differentiated human intestinal cells: an in vitro colonization model.
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霍乱弧菌对培养的分化人肠道细胞的粘附：体外定植模型。
DOI：
10.1128/iai.65.8.3474-3477.1997
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发表时间：
1997
影响因子：
3.1
通讯作者：
Finkelstein,RA
中科院分区：
文献类型：
--
作者：
Bénitez,JA;Spelbrink,RG;Silva,A;Phillips,TE;Stanley,CM;Boesman-Finkelstein,M;Finkelstein,RA
致霍乱弧菌粘附并在高度分化的粘蛋白分泌细胞系HT 29 - 18 N2的单层上增殖。它们的粘附遵循一级动力学，依赖于弧菌的浓度，并被脂多糖部分抑制。转基因弧菌的比较表明，鞭毛，一个活跃的toxR基因，和毒力盒是不是必不可少的初始绑定。血凝素/蛋白酶的失活增加了结合。这种高度分化的人肠细胞系提供了一种多功能的新方法，用于研究肠道定植过程中发生的主要事件：粘附，增殖和分离。
Choleragenic vibrios adhered to and multiplied on monolayers of the highly differentiated mucin-secreting cell line HT29-18N2. Their adherence followed first-order kinetics, was dependent on the concentration of vibrios, and was partially inhibited by lipopolysaccharide. Comparison of genetically modified vibrios showed that flagella, an active toxR gene, and the virulence cassette were not essential for initial binding. Inactivation of the hemagglutinin/protease increased binding. This highly differentiated human intestinal cell line provides a versatile new approach for studying major events occurring during intestinal colonization: adherence, multiplication, and detachment.