Inflammatory response of mice to manufactured titanium dioxide nanoparticles: Comparison of size effects through different exposure routes
Inflammatory response of mice to manufactured titanium dioxide nanoparticles: Comparison of size effects through different exposure routes
复制标题
DOI：
10.1080/17435390701694295
复制
发表时间：
2007-01-01
期刊：
影响因子：
5
通讯作者：
Thorne, Peter S.
中科院分区：
文献类型：
--
作者：
Grassian, Vicki H.;Adamcakova-Dodd, Andrea;Thorne, Peter S.
二氧化钛是一种广泛使用的人造纳米材料，人类接触的机会使得有必要研究其健康影响。使用小鼠炎症模型，研究了滴注和急性吸入暴露于制造商平均粒径为5和21 nm的TiO 2纳米颗粒时炎症反应的尺寸效应。评价了两种尺寸的纳米颗粒的初级纳米颗粒、纳米颗粒团聚体、气溶胶和滴注溶液的性质。小鼠在全身暴露室中或通过鼻滴注急性暴露，并通过计数总细胞和差异细胞、测定BAL液中的总蛋白、LDH活性和炎性细胞因子来评估毒性。还评价了肺的组织病理学变化。结果显示，发现较大的TiO 2纳米颗粒具有中等但显著的毒性。纳米颗粒具有不同的团聚状态，这可能是一个因素
TiO2 is a widely used manufactured nanomaterial and the opportunity for human exposure makes it necessary to study its health implications. Using murine models for inflammation, size effects of inflammatory response in instillation and acute inhalation exposures of TiO2 nanoparticles with manufacturers' average particles sizes of 5 and 21 nm were investigated. The properties of the primary nanoparticles, nanoparticle agglomerates, aerosol and instillation solution for both sized nanoparticles were evaluated. Mice were acutely exposed in a whole-body exposure chamber or through nasal instillation and toxicity was assessed by enumeration of total and differential cells, determination of total protein, LDH activity and inflammatory cytokines in BAL fluid. Lungs were also evaluated for histopathological changes. Results show the larger TiO2 nanoparticles were found to be moderately, but significantly, more toxic. The nanoparticles had different agglomeration states which may be a factor