Antimicrobial peptaibols induce defense responses and systemic resistance in tobacco against tobacco mosaic virus
Antimicrobial peptaibols induce defense responses and systemic resistance in tobacco against tobacco mosaic virus
复制标题
抗菌 peptaibols 诱导烟草对烟草花叶病毒的防御反应和系统抗性
DOI：
10.1111/j.1574-6968.2010.02135.x
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发表时间：
2010-12-01
影响因子：
2.1
通讯作者：
Zhang, Yu-Zhong
中科院分区：
文献类型：
--
作者：
Luo, Yan;Zhang, Dan-Dan;Zhang, Yu-Zhong
木霉菌是众所周知的生物防治剂，因为它们对细菌和真菌植物病原体具有抗微生物活性。然而，其抗病毒活性的生化机制在很大程度上仍然未知。本研究发现，从拟康氏木霉（Trichoderma pseudokoningii）SMF 2中分离的抗菌肽Trichokonins能诱导烟草（Nicotiana tabacum var. Samsun NN）对烟草花叶病毒（TMV）感染的抗性。与对照相比，局部Trichokonin（100 nM）处理导致烟草系统组织中54%的病斑抑制、57%的平均病斑直径减小和30%的平均病斑面积减小，表明Trichokonins诱导烟草对TMV感染的抗性。天花粉蛋白处理增加了烟草中活性氧和酚类化合物的产生。此外，施用毛果菌素显著提高了病程相关酶PAL和POD的活性，并上调了几种植物防御基因的表达。这些结果表明，烟草对TMV的抗性可能与多种防御途径有关。本文报道了肽类抗生素的抗病毒机制，为肽类抗生素在植物病毒病防治中的应用提供了新的思路。
Trichoderma spp. are well-known biocontrol agents because of their antimicrobial activity against bacterial and fungal phytopathogens. However, the biochemical mechanism of their antiviral activity remains largely unknown. In this study, we found that Trichokonins, antimicrobial peptaibols isolated from Trichoderma pseudokoningii SMF2, could induce defense responses and systemic resistance in tobacco (Nicotiana tabacum var. Samsun NN) against tobacco mosaic virus (TMV) infection. Local Trichokonin (100 nM) treatment led to 54% lesion inhibition, 57% reduction in average lesion diameter and 30% reduction in average lesion area in systemic tissue of tobacco compared with control, indicating that Trichokonins induced resistance in tobacco against TMV infection. Trichokonin treatment increased the production of reactive oxygen species and phenolic compounds in tobacco. Additionally, application of Trichokonins significantly increased activities of pathogenesis-related enzymes PAL and POD, and upregulated the expression of several plant defense genes. These results suggested that multiple defense pathways in tobacco were involved in Trichokonin-mediated TMV resistance. We report on the antivirus mechanism of peptaibols, which sheds light on the potential of peptaibols in plant viral disease control.