Detection of seg, seh, and sei genes in Staphylococcus aureus isolates and determination of the enterotoxin productivities of S-aureus isolates harboring seg, seh, or sei genes
Detection of seg, seh, and sei genes in Staphylococcus aureus isolates and determination of the enterotoxin productivities of S-aureus isolates harboring seg, seh, or sei genes
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DOI：
10.1128/jcm.40.3.857-862.2002
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发表时间：
2002-03-01
影响因子：
9.4
通讯作者：
Shinagawa, K
中科院分区：
文献类型：
--
作者：
Omoe, K;Ishikawa, M;Shinagawa, K
为研究金黄色葡萄球菌（Staphylococcus enterotoxin， SE） A To I （SEA To SEI）基因（SEA To SEI）在金黄色葡萄球菌中的分布，采用多重PCR方法对日本食物中毒病例、健康人群、乳腺炎奶牛和牛原料乳中分离的146株葡萄球菌进行了分析。金黄色葡萄球菌1个或多个se基因阳性113株（77.4%）。se基因型可分为14个基因型。seg和sei在同一株金黄色葡萄球菌中共存。新开发的夹心酶联免疫吸附试验表明，大多数含SEH的金黄色葡萄球菌分离株能够产生大量的SEH。然而，大多数携带seg基因的金黄色葡萄球菌分离株和约60%携带sei基因的金黄色葡萄球菌分离株并没有产生可检测水平的seg或sei，而逆转录- pcr分析证实，seg和sei的mrna在携带seg和sei基因的金黄色葡萄球菌菌株中被转录。这些结果表明，定量评估食品中SEG和SEI的产生对于阐明这些新se与食物中毒之间的关系具有重要意义。
To investigate the distribution of staphylococcal enterotoxin (SE) A to I (SEA to SEI) genes (sea to sei) in Staphylococcus aureus, 146 isolates obtained in Japan from humans involved in and samples from food poisoning outbreaks, healthy humans, cows with mastitis, and bovine raw milk were analyzed by multiplex PCR. One hundred thirteen (77.4%) S. aureus isolates were found to be positive for one or more se genes. The se genotype was classified into 14 genotypes. seg and sei coexisted in the same S. aureus strain. The newly developed sandwich enzyme-linked inummosorbent assay showed that most seh-harboring S. aureus isolates were able to produce a significant amount of SEH. However, most of the S. aureus isolates harboring seg and about 60% of the isolates harboring sei did not produce a detectable level of SEG or SEI, while reverse transcription-PCR analysis proved that the mRNAs of SEG and SEI were transcribed in S. aureus strains harboring seg and sei genes. These results suggest the importance of quantitative assessment of SEG and SEI production in foods in order to clarify the relationship between these new SEs and food poisoning.