Metabolic changes in follicular fluid of the dominant follicle in high-yielding dairy cows early post partum.
Metabolic changes in follicular fluid of the dominant follicle in high-yielding dairy cows early post partum.
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DOI：
10.1016/j.theriogenology.2003.12.017
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发表时间：
2004-09-15
期刊：
影响因子：
2.8
通讯作者：
de Kruif, A
中科院分区：
文献类型：
--
作者：
Leroy, JLMR;Vanholder, T;de Kruif, A
排卵前卵母细胞所处的卵泡内环境的特征可能是决定随后生育能力的主要因素之一。我们研究的目的是研究在多大程度上发生在产后早期高产奶牛的代谢变化反映在显性卵泡（bbb8mm）的卵泡液（FF）中。在9头高产奶牛分娩前7天至分娩后46天，每头奶牛采集9份血液样本。从产后第14天起，采用经阴道卵泡抽吸的方法采集同一头奶牛最大卵泡的FF样本，并进行血样采集。使用商业临床和光度化学方法分析血清和FF样品的葡萄糖、对羟基丁酸（β - ohb）、尿素、总蛋白（TP）、甘油三酯（TG）、非酯化脂肪酸（NEFA）和总胆固醇（TC）。试验期内所有奶牛体况均下降（0.94 +/- 0.09点），表现为负能量平衡。与血清相比，FF中葡萄糖浓度显著升高，TP、TG、NEFA和TC浓度显著降低（P < 0.05）。血清和FF中葡萄糖、β - ohb、尿素和TC浓度随时间变化显著（P < 0.05）。在整个研究过程中，血清中所有代谢物的变化都反映在FF的类似变化上。特别是葡萄糖、β - ohb和尿素的相关性非常高。本研究的结果证实，产后不久高产奶牛血清中发现的典型代谢适应反应在卵泡液中，因此可能影响卵母细胞和颗粒细胞的质量。(c) 2004年Elsevier Inc.出版
Characteristics of the intrafollicular environment to which the preovulatory oocyte is exposed may be one of the major factors determining subsequent fertility. The aim of our study was to examine to what extent metabolic changes that occur in early post partum high-yielding dairy cows are reflected in the follicular fluid (FF) of the dominant follicle (> 8 mm). Nine blood samples were taken per cow from nine high-yielding dairy cows between 7 days before and 46 days after parturition. From Day 14 post partum on and together with blood sampling, FF samples of the largest follicle were collected from the same cows by means of transvaginal follicle aspiration. Serum and FF samples were analyzed using commercial clinical and photometric chemistry assays for glucose, P-hydroxybutyrate (beta-OHB), urea, total protein (TP), triglycerides (TG), non-esterified fatty acids (NEFA) and total cholesterol (TC). All cows lost body condition during the experimental period (0.94 +/- 0.09 points) illustrating a negative energy balance during the experimental period. In FF, glucose concentrations were significantly higher and the TP, TG, NEFA and TC concentrations were significantly lower than in serum (P < 0.05). The concentrations of glucose, beta-OHB, urea and TC in serum and in FF changed significantly over time (P < 0.05). Throughout the study, changes of all metabolites in serum were reflected by similar changes in FF. Especially for glucose, beta-OHB and urea the correlations were remarkably high. The results from the present study confirm that the typical metabolic adaptations which can be found in serum of high-yielding dairy cows shortly post partum, are reflected in follicular fluid and, therefore, may affect the quality of both the oocyte and the granulosa cells. (c) 2004 Published by Elsevier Inc.