Response to hormones of cells cultured from human giant cell tumors of bone.
Response to hormones of cells cultured from human giant cell tumors of bone.
复制标题
对从人类骨巨细胞瘤培养的细胞的激素的反应。
DOI：
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发表时间：
1978
影响因子：
5.8
通讯作者：
S. Krane
中科院分区：
文献类型：
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作者：
S. Goldring;J. Dayer;R. Russell;H. Mankin;S. Krane
7例患者骨巨细胞瘤用梭状芽胞杆菌胶原酶和胰酶分散，并在培养中保持贴壁细胞。早期培养物中既有单核细胞，也有多核细胞，推测这些细胞可能来自原始肿瘤的间质细胞和巨细胞。原始多核细胞存活时间不超过7-10天，而单核细胞存活时间较长，可通过胰酶消化传代。在暴露于甲状旁腺激素(PTH)的7种早期培养物中，有5种细胞和培养液在5-10分钟内cAMP水平升高，平均升高约12倍。没有一个细胞对降钙素有反应，在与前列腺素E2孵育后，cAMP有不同程度的上升。在3个肿瘤细胞中，PTH反应随着细胞传代而消失，但在其余2个肿瘤细胞中，PTH反应在多个传代过程中持续存在。甲状旁腺素诱导的cAMP反应在这些细胞中的存在和大小与骨骼起源是一致的。
Giant cell tumors of bone obtained from 7 patients were dispersed with clostridial collagenase and trypsin and adherent cells were maintained in culture. Early cultures contained both mononucleated and multinucleated cells presumably derived from the stromal and giant cells of the original tumor. The original multinucleated cells did not survive for greater than 7-10 days whereas the mononucleated cells persisted and could be passaged by trypsinization. In 5 of 7 early cultures exposed to parathyroid hormone (PTH) there was a rise in cAMP within 5-10 min in both cells and medium which averaged approximately 12-fold. None of the cells responded to calcitonin and a variable rise in cAMP was seen after incubation with prostaglandin E2. In cells cultured from 3 tumors the PTH response disappeared with passage of the cells, but in the remaining 2, PTH response persisted through multiple passages. The presence as well as the magnitude of the PTH-induced cAMP response in these cells is consistent with a skeletal origin.