SPICULE FORMATION IN VITRO BY THE DESCENDANTS OF PRECOCIOUS MICROMERE FORMED AT THE 8‐CELL STAGE OF SEA URCHIN EMBRYO *
SPICULE FORMATION IN VITRO BY THE DESCENDANTS OF PRECOCIOUS MICROMERE FORMED AT THE 8‐CELL STAGE OF SEA URCHIN EMBRYO *
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海胆胚胎 8 细胞阶段形成的早熟微粒后代在体外形成针状体 *
DOI：
10.1111/j.1440-169x.1980.00265.x
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发表时间：
1980
期刊：
影响因子：
4.6
通讯作者：
K. Okazaki
中科院分区：
文献类型：
--
作者：
T. Kitajima;K. Okazaki
海胆胚胎16细胞阶段的微粒体已经被赋予了自我分化成骨针形成细胞的能力（11）。本实验旨在测试这种自我分化所需的因子是否已经定位在8细胞阶段对应于马粪海胆中假定的微节区域的区域中。由于正常胚胎中8细胞期的卵裂球大小相同，因此通过在2细胞期用4 NQO（4-硝基喹啉-1-氧化物）处理或在无钙海水中连续培养，实验诱导了不相等的第三次卵裂，由此小卵裂球被夹向植物极（早熟的微粒）。在含有马血清的天然海水中进行早熟微粒体的离体培养。
The micromeres at the 16‐cell stage of sea urchin embryo have already been endowed with a faculty to self‐differentiate into spicule‐forming cells (11). The present experiment was designed to test whether the factor(s) necessary for such self‐differentiation had already been localized at the 8‐cell stage in an area corresponding to the presumptive micromere region in Hemicentrotus pulcherrimus. Since the blastomeres at the 8‐cell stage are all equal in size in normal embryo, unequal 3rd cleavage, by which small blastomeres are pinched off toward the vegetal pole (precocious micromeres), was experimentally induced either by treatment with 4NQO (4‐nitroquinoline‐1‐oxide) at the 2‐cell stage or by continuous culture in Ca‐free sea water. The precocious micromeres were cultured in vitro in natural sea water containing horse serum.