Lattice light sheet microscopy using tiling lattice light sheets
Lattice light sheet microscopy using tiling lattice light sheets
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DOI：
10.1364/oe.27.001497
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发表时间：
2019-01-21
期刊：
影响因子：
3.8
通讯作者：
Chen, Bi-Chang
中科院分区：
文献类型：
--
作者：
Ga, Liang;Tang, Wei-Chun;Chen, Bi-Chang
我们提出了一种新的方法，用于实现平铺晶格光片显微镜（LLSM）的晶格光片（LLS）在常规LLS显微镜不改变LLS显微镜的硬件。一个LLS平铺通过应用二进制相位图获取从偏离中心的横截面的相应的光学晶格的二进制SLM中使用的LLS显微镜，通过它薄LLS可以平铺图像大的标本，同时保持在整个视场的3D成像能力。我们通过数值模拟和实验研究了该方法，并通过成像嵌入琼脂糖凝胶中的荧光颗粒和LLSM抖动模式下的扩展细胞来证明该方法。(C)根据OSA开放获取出版协议的条款，2019年美国光学学会
We present a novel method used to implement tiling lattice light sheets (LLS) in lattice light sheet microscopy (LLSM) on regular LLS microscopes without changing the LLS microscope hardware. A LLS is tiled by applying binary phase maps acquired from off-center cross-sections of the corresponding optical lattice to the binary SLM used in LLS microscopes, by which a thin LLS can be tiled to image large specimens while maintaining the 3D imaging ability in the entire field of view. We investigate the method via numerical simulations and experiments, and demonstrate the method by imaging fluorescent particles embedded in agarose gel and expanded cells in the dithered mode of LLSM. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement