Cellular localization of mGluR3 and mGluR5 mRNAs in normal and injured rat brain
Cellular localization of mGluR3 and mGluR5 mRNAs in normal and injured rat brain
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DOI：
10.1016/j.brainres.2007.02.041
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发表时间：
2007-05-29
期刊：
影响因子：
2.9
通讯作者：
Condorelli, Daniele F.
中科院分区：
文献类型：
--
作者：
Mudo, Giuseppa;Trovato-Salinaro, Angela;Condorelli, Daniele F.
为了了解代谢型谷氨酸受体（mGluRs）在大脑中的作用，重要的是要知道mGluRs是如何在不同的细胞类型之间差异表达。目前，mGluR 3和mGluR 5的细胞表达主要在蛋白质方面进行研究，观察结果表明mGluR 3和mGluR 5在神经元和神经胶质细胞中表达。为了验证正常和损伤脑中表达mGluR 3和mGluR 5 mRNA的脑细胞类型，我们通过原位杂交mGluR 3或mGluR 5 mRNA和免疫组织化学特异性细胞标记物进行了双标记分析。这种方法使我们能够发现mGluR 3 mRNA在神经元（NeuN阳性细胞）和神经胶质细胞中表达，如星形胶质细胞（GFAP阳性细胞）和少突胶质细胞（CNPase-阳性细胞）。同样的分析表明，只有NeuN阳性细胞表达mGluR 5 mRNA。在海马结构损伤（红藻氨酸诱导的癫痫发作或鹅膏蕈氨酸注射）两种模型中，mGluR 3 mRNA表达的时间过程显示，损伤区域mGluR 3的表达增加。这种作用出现在损伤后1周，并定位于GFAP和CNPase-positive细胞。而mGluR 5在病变区无表达。目前的研究结果有助于扩展现有的数据细胞类型表达mGluR 3和mGluR 5在正常和受伤的大脑，并可能是相关的了解机制，驱动神经元细胞的相互作用，在正常和修复过程。(c)2007 Elsevier B. V.保留所有权利。
In order to understand the role of metabotropic glutamate receptors (mGluRs) in the brain, it is important to know how the mGluRs are differentially expressed among the different cell types. At present, the cellular expression of mGluR3 and mGluR5 has been mostly studied in terms of proteins with observations suggesting the expression of both mGluR3 and mGluR5 in neuronal and in glial cells. in order to verify the brain cell type-expressing mGluR3 and mGluR5 mRNAs, both in normal and injured brain, we performed a double labeling analysis, by in situ hybridization for mGluR3 or mGluR5 mRNA and immunohistochemistry for specific cellular markers. This approach allowed us to find mGluR3 mRNA expressed in neurons (NeuN-positive cells), and in glial cells, such as astrocytes (GFAP-positive cells) and oligodendrocytes (CNPase-positive cells). The same analysis showed that only NeuN-positive cells express mGluR5 mRNA. The time course of mGluR3 mRNA expression in two models of hippocampal formation lesion, kainate-induced seizures or ibotenic acid injection, showed an increased expression of mGluR3 in the area of lesion. This effect appears 1 week after the injury and was localized in GFAP- and CNPase-positive cells. In contrast, mGluR5 was not found expressed in the area of lesion. The present results contribute to extend available data on cell type-expressing mGluR3 and mGluR5 in normal and injured brain and could be relevant to understand the mechanisms that drive neuronglial cells interaction both in normal and repairing processes. (c) 2007 Elsevier B.V. All rights reserved.